David, Charles N.
A quantitative method for maceration of hydra tissue.
In: Development Genes and Evolution, Vol. 171, No. 4: pp. 259-268
A method is described for the maceration (dissociation) of hydra tissue into single cells. The cells have characteristic morphology such that all basic types — epithelial, gland, mucous, interstitial, nematoblast, and nerve — can be distinguished. Criteria are given for identifying each cell type by phase contrast microscopy. It is shown that maceration quantitatively recovers cells from hydra tissue.