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Rüffer, Martina and Zenk, Meinhart H. (1994): Canadine synthase from Thalictrum tuberosum cell cultures catalyses the formation of the methylenedioxy bridge in berberine synthesis. In: Phytochemistry, Vol. 36, No. 5: pp. 1219-1223
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Abstract

An enzyme system catalysing the formation of the methylenedioxy bridge at ring A of (S)-canadine [ = (S)-tetrahydroberberine] from (S)-tetrahydrocolumbamine has been detected in microsomal preparations from different Ranunculaceae and Berberidaceae cell cultures. The cytochrome P-450 enzyme complex has been partly characterized from a protoberberine alkaloid producing Thalictrum tuberosum L. cell line. The enzyme complex consisting of a microsomal associated oxidase with a cytochrome P-450 reductase has a pH optimum at pH 8.5 and a temperature optimum of 40°. The apparent Km values are 33 μM for NADPH and 11.5 μM for tetrahydrocolumbamine.