Rüffer, Martina and Zenk, Meinhart H.
Enzymatic formation of protopines by a microsomal cytochrome P-450 system of Corydalis vaginans.
In: Tetrahedron Letters, Vol. 28, No. 44: pp. 5307-5310
A microsomal cytochrome P-450-NADPH dependent enzyme which hydroxylates stereo- and regiospecifically carbon atom 14 of (S)- -N- methyltetrahydroprotoberberines has been discovered in a number of plant cell cultures originating from species containing protopine alkaloids; the monooxygenase was solubilized, partially purified (100-fold) and characterized.