Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus

Rapp, Maria and Lucin, Pero and Messerle, Martin and Loh, Lambert C. and Koszinowski, Ulrich H. (1994): Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus. In: Journal of General Virology, Vol. 75: pp. 183-188

Abstract

The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb, which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity of the MCMV gH with a 87K envelope glycoprotein described previously by Loh and Qualtiere. Immunization of mice with the Vac-gH recombinant gave rise to an anti-gH serum, which neutralized MCMV without complement in vitro.

• Item Type: Article
• Collections: Medicine
• Subjects: 600 Technology > 610 Medicine and health