Cellular distribution and amount of chromogranin A in bovine endocrine pancreas

Ehrhart, M. and Jörns, A. and Grube, D. and Gratzl, Manfred (1988): Cellular distribution and amount of chromogranin A in bovine endocrine pancreas. In: Journal of Histochemistry & Cytochemistry, Vol. 36, No. 5: pp. 467-472

Abstract

We determined the cellular distribution and the amount of chromogranin A in endocrine cells of bovine pancreas using a polyclonal antibody against bovine adrenomedullary chromogranin A. The relative amounts of chromogranin A in the different cells of the endocrine pancreas were determined by computer-assisted analyses of the optical densities of the immunoreactivities in the stained sections. More than 80% of the immunoreactive chromogranin A was located in the pancreatic B-cells. In immunoblots of acid tissue extracts, only one chromogranin A band (MW 74 KD) was observed. Quantification of the immunoblots revealed that 3 micrograms of chromogranin A and 918 micrograms of insulin were present per gram pancreas (wet weight), equivalent to a molar ratio of 460 mumol chromogranin A per mol insulin.

• Item Type: Article
• Collections: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• Signature: UBM:0760/4 Med.GZ 194(36
• Language: English