Evolutionary analysis identifies a Golgi pathway and correlates lineage-specific factors with endomembrane organelle emergence in apicomplexans

Klinger, Christen M.; Jimenez-Ruiz, Elena; Mourier, Tobias; Klingl, Andreas; Lemgruber, Leandro; Pain, Arnab; Dacks, Joel B. und Meissner, Markus (2024): Evolutionary analysis identifies a Golgi pathway and correlates lineage-specific factors with endomembrane organelle emergence in apicomplexans. In: Cell Reports, Vol. 43, No. 2, 113740

Abstract

The organelle paralogy hypothesis (OPH) aims to explain the evolution of non-endosymbiotically derived organelles. It predicts that lineage-specific pathways or organelles should result when identity-encoding membrane-trafficking components duplicate and co-evolve. Here, we investigate the presence of such lineagespecific membrane-trafficking machinery paralogs in Apicomplexa, a globally important parasitic lineage. We are able to identify 18 paralogs of known membrane-trafficking machinery, in several cases co-incident with the presence of new endomembrane organelles in apicomplexans or their parent lineage, the Alveolata. Moreover, focused analysis of the apicomplexan Arf-like small GTPases (i.e., ArlX3) revealed a specific postGolgi trafficking pathway. This pathway appears involved in delivery of proteins to micronemes and rhoptries, with knockdown demonstrating reduced invasion capacity. Overall, our data have identified an unforeseen post-Golgi trafficking pathway in apicomplexans and are consistent with the OPH mechanism acting to produce endomembrane pathways or organelles at various evolutionary stages across the alveolate lineage.

• Item Type: Journal article
• Faculties: Veterinary Medicine > Department of Veterinary Medicine; Biology > Department Biology I
• Subjects: 500 Science > 570 Life sciences; biology; 500 Science > 590 Animals (zoology); 600 Technology > 610 Medicine and health
• ISSN: 22111247
• Language: English
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892