ORCID: https://orcid.org/0000-0002-8861-9224; Rehn, Fabian ORCID: https://orcid.org/0000-0001-8815-0684; Pils, Marlene ORCID: https://orcid.org/0000-0002-1506-3675; Kraemer-Schulien, Victoria ORCID: https://orcid.org/0000-0002-7413-7646; Cousin, Anneliese; Kutzsche, Janine ORCID: https://orcid.org/0000-0003-0271-7390; Bujnicki, Tuyen ORCID: https://orcid.org/0000-0002-7191-8455; Freiesleben, Silka D. ORCID: https://orcid.org/0000-0002-2141-8671; Schneider, Luisa-Sophie ORCID: https://orcid.org/0000-0001-5822-1744; Preis, Lukas; Priller, Josef; Spruth, Eike J.; Altenstein, Slawek; Schneider, Anja ORCID: https://orcid.org/0000-0001-9540-8700; Fliessbach, Klaus; Wiltfang, Jens ORCID: https://orcid.org/0000-0003-1492-5330; Hansen, Niels ORCID: https://orcid.org/0000-0001-5785-9594; Rostamzadeh, Ayda; Düzel, Emrah; Glanz, Wenzel; Incesoy, Enise I. ORCID: https://orcid.org/0000-0003-2014-4098; Buerger, Katharina ORCID: https://orcid.org/0000-0002-5898-9953; Janowitz, Daniel ORCID: https://orcid.org/0009-0003-4090-547X; Ewers, Michael; Perneczky, Robert ORCID: https://orcid.org/0000-0003-1981-7435; Rauchmann, Boris-Stephan ORCID: https://orcid.org/0000-0003-4547-6240; Teipel, Stefan; Kilimann, Ingo; Laske, Christoph; Munk, Matthias H.; Spottke, Annika; Roy, Nina; Heneka, Michael T. ORCID: https://orcid.org/0000-0003-4996-1630; Brosseron, Frederic ORCID: https://orcid.org/0000-0003-3137-7516; Wagner, Michael ORCID: https://orcid.org/0000-0003-2589-6440; Roeske, Sandra; Ramirez, Alfredo ORCID: https://orcid.org/0000-0003-4991-763X; Schmid, Matthias; Jessen, Frank; Bannach, Oliver; Peters, Oliver und Willbold, Dieter ORCID: https://orcid.org/0000-0002-0065-7366
(2024):
Blood-based quantification of Aβ oligomers indicates impaired clearance from brain in ApoE ε4 positive subjects.
In: Communications Medicine, Vol. 4, 262
[PDF, 2MB]
Abstract
Background
Quantification of Amyloid beta (Aβ) oligomers in plasma enables early diagnosis of Alzheimer’s Disease (AD) and improves our understanding of underlying pathologies. However, quantification necessitates an extremely sensitive and selective technology because of very low Aβ oligomer concentrations and possible interference from matrix components.
Methods
In this report, we developed and validated a surface-based fluorescence distribution analysis (sFIDA) assay for quantification of Aβ oligomers in plasma.
Results
The blood-based sFIDA assay delivers a sensitivity of 1.8 fM, an inter- and intra-assay variation below 20% for oligomer calibration standards and no interference with matrix components. Quantification of Aβ oligomers in 359 plasma samples from the DELCODE cohort reveals lower oligomer concentrations in subjective cognitive decline and AD patients than healthy Control participants.
Conclusions
Correlation analysis between CSF and plasma oligomer concentrations indicates an impaired clearance of Aβ oligomers that is dependent on the ApoE ε4 status.
| Item Type: | Journal article |
|---|---|
| Faculties: | Medicine > Munich Cluster for Systems Neurology (SyNergy) Medicine > Institute for Stroke and Dementia Research (ISD) Medicine > Medical Center of the University of Munich > Clinic and Polyclinic for Psychiatry and Psychotherapy |
| Subjects: | 600 Technology > 610 Medicine and health |
| URN: | urn:nbn:de:bvb:19-epub-123293-0 |
| ISSN: | 2730-664X |
| Language: | English |
| Item ID: | 123293 |
| Date Deposited: | 23. Dec 2024 11:58 |
| Last Modified: | 23. Dec 2024 11:58 |
| DFG: | Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198 |
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