ORCID: https://orcid.org/0000-0002-0412-2502; Wu, Jiaojiao ORCID: https://orcid.org/0009-0003-7609-0938; Corvaglia, Valentina ORCID: https://orcid.org/0000-0002-6180-5975; Allmendinger, Lars ORCID: https://orcid.org/0000-0002-9476-6175; Scheckenbach, Michael ORCID: https://orcid.org/0000-0002-7912-6292; Tinnefeld, Philip ORCID: https://orcid.org/0000-0003-4290-7770 und Huc, Ivan ORCID: https://orcid.org/0000-0001-7036-9696
(2025):
DNA Mimic Foldamer Recognition of a Chromosomal Protein.
In: Angewandte Chemie International Edition, Vol. 64, No. 8, e202422958
[PDF, 1MB]
Abstract
Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. The foldamers were shown to bind to Sac7d better than a DNA duplex of comparable length. The interaction is diastereoselective and takes place at the DNA binding site. Crystallography revealed that the DNA mimic foldamers have a binding mode of their own and that they can bind to Sac7d without being kinked.
| Item Type: | Journal article |
|---|---|
| EU Funded Grant Agreement Number: | ERC-2021-ADG-320892 |
| EU Projects: | Horizon Europe > ERC Grants |
| Faculties: | Chemistry and Pharmacy > Department of Pharmacy |
| Subjects: | 500 Science > 540 Chemistry |
| URN: | urn:nbn:de:bvb:19-epub-126548-8 |
| ISSN: | 1433-7851 |
| Language: | English |
| Item ID: | 126548 |
| Date Deposited: | 13. Jun 2025 05:58 |
| Last Modified: | 13. Jun 2025 05:58 |
| DFG: | Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 325871075 |
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