Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

Eckstein, Yasmin; Kessler, Barbara; Hinrichs, Arne ; Novak, Istvan; Thaden, Anne von; Lorenzen, Timo; Rathkolb, Birgit; Scholz, Armin; Blutke, Andreas; Koopmans, Sietse-Jan; Angelis, Martin Hrabĕ de; Christoffersen, Berit Østergaard; Wolf, Eckhard und Renner, Simone (2025): Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. In: STAR Protocols, Vol. 6, No. 2, 103774

Abstract

The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis.

For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2

• Item Type: Journal article
• Faculties: Veterinary Medicine
• Subjects: 500 Science > 590 Animals (zoology)
• ISSN: 26661667
• Language: English
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 536691227