Analyzing mitochondrial respiration of human induced pluripotent stem cell-derived myeloid progenitors using Seahorse technology

Fan, Yanxin; Mizoguchi, Yoko; Tatematsu, Megumi; Linder, Monika I.; Frenz, Stephanie; Choi, Jongsu und Klein, Christoph (2023): Analyzing mitochondrial respiration of human induced pluripotent stem cell-derived myeloid progenitors using Seahorse technology. In: STAR Protocols, Vol. 4, No. 1: p. 102073

Abstract

Mitochondrial metabolism is critical in hematopoietic stem cell maintenance and differentiation. Here, we present a step-by-step protocol to efficiently differentiate human induced pluripotent stem cells into myeloid progenitors by a robust feeder- and serum-free system. Furthermore, we provide a protocol to subsequently assess mitochondrial function in iPSC-derived myeloid progenitors. We comprehensively describe a protocol to analyze and to quantify key parameters of mitochondrial respiration of iPSC-derived myeloid progenitors by the Seahorse XFe96 Analyzer. Additionally, our protocol includes extensive troubleshooting suggestions.

• Item Type: Journal article
• Form of publication: Publisher's Version
• Faculties: Medicine > Medical Center of the University of Munich > Paediatric Surgery Clinic and Polyclinic in the Dr. von Hauner Children's Hospital
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 26661667
• Language: English
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 165054336
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892