Gardner, A.; Odebralski, J.; Zahler, Stefan; Korman, R. Z.; Aronson, A. I.
Glutamine synthetase subunit mixing and regulation in Bacillus subtilis partial diploids.
In: Journal of Bacteriology, Vol. 149, No. 1: pp. 378-380
A specialized transducing phage, SPβ c2 dglnA2, of Bacillus subtilis was used to construct partial diploids with various glutamine auxotrophs. The overproduction of manganese-stimulated glutamine synthetase no longer occurred in the diploids. The kinetics of heat inactivation of the enzyme extracted from two diploids suggests that there was subunit mixing.