Viola, Stefania; Rühle, Thilo; Leister, Dario: A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803. In: Microbial Cell Factories 2014, 13:4




The cyanobacterium Synechocystis sp. PCC 6803 is widely used for research on photosynthesis and circadian rhythms, and also finds application in sustainable biotechnologies. Synechocystis is naturally transformable and undergoes homologous recombination, which enables the development of a variety of tools for genetic and genomic manipulations. To generate multiple gene deletions and/or replacements, marker-less manipulation methods based on counter-selection are generally employed. Currently available methods require two transformation steps with different DNA plasmids.