Serdjukow, Sascha; Kink, F.; Steigenberger, B.; Tomas-Gamasa, Maria; Carell, Thomas
(20. December 2013):
Synthesis of gamma-labeled nucleoside 5 `-triphosphates using click chemistry.
In: Chemical communications, Vol. 50, No. 15: pp. 1861-1863
Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of gamma-alkyne modified triphosphate amidates that are converted into a variety of gamma-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.