Weiss, Elisabeth H.; Golden, L.; Zakut, A.; Mellor, Andrew L.; Fahrner, K.; Kvist, S.; Flavell, Richard A.
The DNA sequence of the H-2Kb gene. Evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.
In: EMBO Journal, Vol. 2: S. 453-462
We have determined the DNA sequence of the H-2Kb gene of
the C57B1/10 mouse. Comparison of this sequence with that
of the allelic H-2Kd shows surprisingly that the exons have accumulated
more mutations than their introns. Moreover,
many of these changes in the exons are clustered in short
regions or hot spots. Additional comparison of these sequences
with the H-2Ld and H-2Db sequences shows that, in
several cases, the altered sequence generated at the hot spot is
identical to the corresponding region of a non-allelic H-2
gene. The clustered changes are responsible for 60wo of the
amino acid differences between the H-2Kb and H-2Kd genes
and suggest that micro-gene conversion events occurring
within the exons and involving only tens of nucleotides are an
important mechanism for the generation of polymorphic differences
between natural H-2 alleles.