Polland, Hans-Joachim; Franz, M. A.; Zinth, Wolfgang; Kaiser, Wolfgang; Hegemann, P.; Oesterhelt, Dieter
Picosecond events in the photochemical cycle of the light-driven chloride-pump halorhodopsin.
In: Biophysical Journal, No. 47: pp. 55-59
The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time a red-shifted photoproduct is formed that remains stable for at least 2 ns.