%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Matthew H. Van Dam, Athena W. Lam, Katayo Sagata, Bradley Gewa, Raymond Laufa, Michael Balke, Brant C. Faircloth, Alexander Riedel
Ultraconserved elements (UCEs) resolve the phylogeny of Australasian smurf-weevils
www.plosone.org
www.plosone.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 12 0 R/Annots 13 0 R/Contents 14 0 R/TrimBox[0 0 612 792]>>
endobj
13 0 obj
[15 0 R 16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R]
endobj
15 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref004)>>
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref005)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref006)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref009)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref010)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref011)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref012)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref010)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref002)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref013)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref014)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref015)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref016)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.g001)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.g001)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref017)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref018)>>
endobj
32 0 obj
<>/Border[0 0 0]/Dest(Rpone.0188044.ref002)>>
endobj
33 0 obj
<>/Border[0 0 0]/A 34 0 R>>
endobj
34 0 obj
<>
endobj
14 0 obj
[35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 43 0 R 44 0 R 45 0 R 46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R]
endobj
35 0 obj
<>stream
q
0.83 0.64 0.02 0 k
249.5055 693.2976 m
254.2677 693.2976 l
h
f*
487.8992 615.3449 m
492.6614 615.3449 l
h
f*
516.4157 589.3228 m
521.178 589.3228 l
h
f*
526.337 589.3228 m
531.0992 589.3228 l
h
f*
221.1024 550.3181 m
230.5701 550.3181 l
h
f*
232.8378 550.3181 m
242.3622 550.3181 l
h
f*
543.515 537.3354 m
553.0394 537.3354 l
h
f*
555.3071 537.3354 m
564.7748 537.3354 l
h
f*
269.1213 485.348 m
273.8835 485.348 l
h
f*
276.1512 485.348 m
285.6756 485.348 l
h
f*
379.7291 407.3386 m
389.2535 407.3386 l
h
f*
391.5213 407.3386 m
401.0457 407.3386 l
h
f*
279.7228 381.3165 m
289.1906 381.3165 l
h
f*
314.8724 368.3339 m
334.4315 368.3339 l
h
f*
422.0787 316.3465 m
441.6378 316.3465 l
h
f*
450.652 316.3465 m
460.1764 316.3465 l
h
f*
462.4441 316.3465 m
471.9118 316.3465 l
h
f*
399.7984 151.1433 m
404.5606 151.1433 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(a)Tj
0.6519 0 Td
(set)Tj
1.3039 0 Td
(of)Tj
1.0148 0 Td
(target)Tj
2.5115 0 Td
(enrichment)Tj
4.8699 0 Td
(baits)Tj
2.0863 0 Td
(designed)Tj
3.7644 0 Td
(to)Tj
1.0261 0 Td
(enrich)Tj
2.7836 0 Td
(ultraconserved)Tj
6.1398 0 Td
(elements)Tj
3.7644 0 Td
(\(UCEs\))Tj
3.2258 0 Td
(across)Tj
-33.1424 -1.3039 Td
(Coleoptera)Tj
4.6091 0 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(],)Tj
1.6044 0 Td
(and)Tj
1.7291 0 Td
(we)Tj
1.3266 0 Td
(use)Tj
1.5307 0 Td
(the)Tj
1.4683 0 Td
(resulting)Tj
3.7304 0 Td
(sequence)Tj
3.8608 0 Td
(data)Tj
1.9105 0 Td
(to)Tj
1.0261 0 Td
(examine)Tj
3.6 0 Td
(the)Tj
1.474 0 Td
(phylogeny)Tj
4.3653 0 Td
(of)Tj
1.0148 0 Td
(Austral-)Tj
-33.2501 -1.2982 Td
(asian)Tj
2.262 0 Td
(smurf-weevils)Tj
5.7996 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(tribe)Tj
2.0863 0 Td
(Eupholini.)Tj
4.422 0 Td
(We)Tj
1.6101 0 Td
(had)Tj
1.7121 0 Td
(two)Tj
1.7065 0 Td
(main)Tj
2.2904 0 Td
(goals:)Tj
2.4604 0 Td
(1\))Tj
1.0318 0 Td
(to)Tj
1.0262 0 Td
(examine)Tj
3.5999 0 Td
(the)Tj
1.474 0 Td
(utility)Tj
2.5795 0 Td
(of)Tj
-36.5666 -1.2983 Td
(these)Tj
2.2506 0 Td
(newly)Tj
2.5796 0 Td
(designed)Tj
3.7643 0 Td
(universal)Tj
3.8551 0 Td
(Coleoptera)Tj
4.6091 0 Td
(UCE)Tj
2.1884 0 Td
(baits)Tj
2.0863 0 Td
(for)Tj
1.3889 0 Td
(phylogenetic)Tj
5.3235 0 Td
(studies)Tj
2.9877 0 Td
(of)Tj
1.0148 0 Td
(weevils,)Tj
3.3051 0 Td
(using)Tj
-35.3534 -1.2983 Td
(freshly)Tj
2.8913 0 Td
(collected)Tj
3.7417 0 Td
(individuals)Tj
4.6488 0 Td
(as)Tj
1.0148 0 Td
(well)Tj
1.8198 0 Td
(as)Tj
1.0148 0 Td
(older)Tj
2.296 0 Td
(museum)Tj
3.6964 0 Td
(specimens)Tj
4.3653 0 Td
(\(up)Tj
1.6044 0 Td
(to)Tj
1.0262 0 Td
(65)Tj
1.1735 0 Td
(years\);)Tj
2.829 0 Td
(and)Tj
1.7291 0 Td
(2\))Tj
1.0374 0 Td
(to)Tj
1.0262 0 Td
(test)Tj
-35.9147 -1.3039 Td
(the)Tj
1.4683 0 Td
(monophyly)Tj
4.8018 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(three)Tj
2.2677 0 Td
(main)Tj
2.2904 0 Td
(genera)Tj
2.8856 0 Td
(within)Tj
2.812 0 Td
(the)Tj
1.4683 0 Td
(Eupholini,)Tj
4.422 0 Td
(examining)Tj
4.4617 0 Td
(the)Tj
1.4684 0 Td
(validity)Tj
3.1691 0 Td
(of)Tj
1.0148 0 Td
(previ-)Tj
-35.0133 -1.2982 Td
(ous)Tj
1.6157 0 Td
(ecomorphology-driven)Tj
9.4393 0 Td
(classification)Tj
5.3461 0 Td
(efforts.)Tj
-15.2049 -1.2983 Td
(Ultraconserved)Tj
6.3495 0 Td
(genomic)Tj
3.6737 0 Td
(elements)Tj
3.7644 0 Td
(\(UCEs,)Tj
/F11 1 Tf
3.1181 0 Td
[()-3()2()10()]TJ
/F7 1 Tf
2.3244 0 Td
(Faircloth)Tj
/F11 1 Tf
3.8211 0 Td
[()8()]TJ
0.9127 0 Td
[()11()]TJ
/F7 1 Tf
(.)Tj
1.1679 0 Td
(2012)Tj
2.1203 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(]\),)Tj
1.9445 0 Td
(provide)Tj
3.2882 0 Td
(a)Tj
0.652 0 Td
(power-)Tj
-34.333 -1.3039 Td
(ful)Tj
1.2812 0 Td
(approach)Tj
3.9515 0 Td
(to)Tj
1.0261 0 Td
(sequence)Tj
3.8551 0 Td
(many)Tj
2.4775 0 Td
(independent)Tj
5.278 0 Td
(regions)Tj
3.1635 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(genome.)Tj
3.634 0 Td
(UCEs)Tj
2.5455 0 Td
(have)Tj
2.0636 0 Td
(proven)Tj
3.0387 0 Td
(useful)Tj
-34.7978 -1.2983 Td
(in)Tj
1.0318 0 Td
(resolving)Tj
3.8664 0 Td
(phylogenies)Tj
4.9606 0 Td
(at)Tj
0.9524 0 Td
(multiple)Tj
3.566 0 Td
(phylogenetic)Tj
5.3234 0 Td
(scales)Tj
2.4718 0 Td
(both)Tj
2.0579 0 Td
(shallow)Tj
3.2259 0 Td
(and)Tj
1.7291 0 Td
(deep)Tj
2.1146 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(].)Tj
2.5909 0 Td
(UCEs,)Tj
-33.8908 -1.2983 Td
(like)Tj
1.6497 0 Td
(many)Tj
2.4775 0 Td
(other)Tj
2.347 0 Td
(reduced)Tj
3.4469 0 Td
(representation)Tj
6.0151 0 Td
(techniques)Tj
4.5241 0 Td
(that)Tj
1.7801 0 Td
(rely)Tj
1.7178 0 Td
(on)Tj
1.2756 0 Td
(oligonucleotide)Tj
6.4063 0 Td
(bait)Tj
2.5114 0 Td
(capture)Tj
-34.1515 -1.3039 Td
(procedures,)Tj
4.8982 0 Td
(also)Tj
1.7688 0 Td
(have)Tj
2.0579 0 Td
(the)Tj
1.4684 0 Td
(potential)Tj
3.7644 0 Td
(to)Tj
1.0261 0 Td
(perform)Tj
3.5149 0 Td
(well)Tj
1.8199 0 Td
(when)Tj
2.4037 0 Td
(collecting)Tj
4.0706 0 Td
(data)Tj
1.9162 0 Td
(from)Tj
2.2053 0 Td
(museum)Tj
3.6964 0 Td
(sam-)Tj
-34.6108 -1.2982 Td
(ples)Tj
1.7688 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
3.2484 0 Td
(Given)Tj
2.6306 0 Td
(the)Tj
1.474 0 Td
(challenges)Tj
4.3143 0 Td
(with)Tj
2.0012 0 Td
(obtaining)Tj
4.0592 0 Td
(samples)Tj
3.3846 0 Td
(for)Tj
1.3833 0 Td
(many)Tj
2.4774 0 Td
(rare)Tj
1.8199 0 Td
(taxa)Tj
1.8538 0 Td
(and/or)Tj
2.9367 0 Td
(from)Tj
-33.3522 -1.2983 Td
(logistically)Tj
4.4333 0 Td
(challenging)Tj
4.8189 0 Td
(regions)Tj
3.1634 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(world,)Tj
2.7836 0 Td
(such)Tj
2.0579 0 Td
(approaches)Tj
4.7395 0 Td
(can)Tj
1.6214 0 Td
(be)Tj
1.1509 0 Td
(highly)Tj
2.7099 0 Td
(beneficial)Tj
4.0478 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(].)Tj
-34.0098 -1.3039 Td
(The)Tj
1.7801 0 Td
(challenge)Tj
3.9571 0 Td
(of)Tj
1.0148 0 Td
(obtaining)Tj
4.0649 0 Td
(weevil)Tj
2.7212 0 Td
(samples)Tj
3.3789 0 Td
(is)Tj
0.8504 0 Td
(even)Tj
2.0693 0 Td
(more)Tj
2.3414 0 Td
(complex)Tj
3.6226 0 Td
(because)Tj
3.3222 0 Td
(many)Tj
2.4775 0 Td
(species)Tj
2.9933 0 Td
(can)Tj
1.6271 0 Td
(be)Tj
-36.2208 -1.2983 Td
(found)Tj
2.6191 0 Td
(only)Tj
1.9729 0 Td
(on)Tj
1.2756 0 Td
(specific)Tj
3.1918 0 Td
(host)Tj
1.9162 0 Td
(plants)Tj
2.6249 0 Td
(at)Tj
0.9524 0 Td
(very)Tj
1.9219 0 Td
(particular)Tj
4.1102 0 Td
(times)Tj
2.3924 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(year,)Tj
2.1316 0 Td
(and)Tj
1.7291 0 Td
(many)Tj
2.4775 0 Td
(weevil)Tj
2.7156 0 Td
(hosts)Tj
-34.5144 -1.2982 Td
(are)Tj
1.4456 0 Td
(unknown.)Tj
4.3086 0 Td
(In)Tj
1.1112 0 Td
(addition)Tj
3.5943 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(challenges)Tj
4.32 0 Td
(of)Tj
1.0148 0 Td
(sampling,)Tj
4.1045 0 Td
(many)Tj
2.4775 0 Td
(weevil)Tj
2.7212 0 Td
(groups)Tj
2.965 0 Td
(have)Tj
2.0636 0 Td
(undergone)Tj
-32.6208 -1.2983 Td
(rapid)Tj
2.3357 0 Td
(radiations)Tj
4.2349 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(])Tj
2.5512 0 Td
(necessitating)Tj
5.3404 0 Td
(the)Tj
1.474 0 Td
(development)Tj
5.4085 0 Td
(of)Tj
1.0148 0 Td
(large)Tj
2.16 0 Td
(genome-wide)Tj
5.6579 0 Td
(data)Tj
1.9162 0 Td
(sets)Tj
1.6611 0 Td
(to)Tj
1.0261 0 Td
(take)Tj
-34.7808 -1.3039 Td
(on)Tj
1.2755 0 Td
(such)Tj
2.058 0 Td
(challenges.)Tj
-2.1373 -1.2983 Td
(The)Tj
1.7858 0 Td
(Eupholini)Tj
4.1952 0 Td
(represent)Tj
3.9628 0 Td
(a)Tj
0.652 0 Td
(tribe)Tj
2.0863 0 Td
(of)Tj
1.0148 0 Td
(weevils)Tj
3.0784 0 Td
(with)Tj
2.0012 0 Td
(approximately)Tj
5.9754 0 Td
(300)Tj
1.6441 0 Td
(known)Tj
3.0047 0 Td
(species.)Tj
3.2258 0 Td
(New)Tj
-33.8227 -1.2983 Td
(Guinea)Tj
3.135 0 Td
(is)Tj
0.8504 0 Td
(the)Tj
1.4684 0 Td
(Eupholini's)Tj
4.7735 0 Td
(center)Tj
2.7099 0 Td
(of)Tj
1.0148 0 Td
(diversity)Tj
3.6453 0 Td
(both)Tj
2.058 0 Td
(in)Tj
1.0374 0 Td
(terms)Tj
2.4945 0 Td
(of)Tj
1.0148 0 Td
(species)Tj
2.9934 0 Td
(and)Tj
1.7291 0 Td
(genera.)Tj
3.1124 0 Td
(Currently,)Tj
-32.0369 -1.3039 Td
(there)Tj
2.262 0 Td
(are)Tj
1.4513 0 Td
(three)Tj
2.262 0 Td
(main)Tj
2.2904 0 Td
(genera)Tj
2.8857 0 Td
(recognized)Tj
4.5921 0 Td
(within)Tj
2.8119 0 Td
(the)Tj
1.4684 0 Td
(Eupholini,)Tj
4.422 0 Td
(although)Tj
3.7587 0 Td
(the)Tj
1.4683 0 Td
(monophyly)Tj
4.8019 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
-35.4895 -1.2982 Td
(is)Tj
0.8447 0 Td
(somewhat)Tj
4.2746 0 Td
(questionable:)Tj
/F11 1 Tf
5.4992 0 Td
[()16()10( )10(\n)6()10()12()-4()]TJ
/F7 1 Tf
3.8381 0 Td
(Boisduval)Tj
4.1215 0 Td
(\(63)Tj
1.5137 0 Td
(species\),)Tj
/F11 1 Tf
3.5603 0 Td
[()18(\r)-13()18()6()11( )10(\n)6()10()12()-4()]TJ
/F7 1 Tf
5.6409 0 Td
(Heller)Tj
2.6986 0 Td
(\(76)Tj
1.5137 0 Td
(species\))Tj
-33.5053 -1.2983 Td
(and)Tj
/F11 1 Tf
1.7291 0 Td
[()-4(\n)18()12()6()7()-3()7()12( )10(\n)6()]TJ
/F7 1 Tf
5.2327 0 Td
(Montrouzier)Tj
5.3461 0 Td
(\(137)Tj
1.9899 0 Td
(species\))Tj
3.3335 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
/F11 1 Tf
3.2542 0 Td
[()16()10( )10(\n)6()10()12()-4()]TJ
/F7 1 Tf
3.8381 0 Td
(species)Tj
2.999 0 Td
(are)Tj
1.4457 0 Td
(brilliantly)Tj
4.1102 0 Td
(colored)Tj
-33.2785 -1.3039 Td
(and)Tj
1.7291 0 Td
(can)Tj
1.627 0 Td
(be)Tj
1.1509 0 Td
(divided)Tj
3.2145 0 Td
(in)Tj
1.0374 0 Td
(two)Tj
1.7065 0 Td
(morphologically)Tj
6.7634 0 Td
(distinct)Tj
3.2088 0 Td
(groups,)Tj
3.1975 0 Td
(i.e.)Tj
1.3606 0 Td
(the)Tj
/F11 1 Tf
1.4683 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F11 1 Tf
0.9922 0 Td
[()-3()13(\n)6()4()2()10(\n)6()2()-9()-9()]TJ
/F7 1 Tf
(-group)Tj
7.4437 0 Td
(and)Tj
-34.8999 -1.2983 Td
(the)Tj
/F11 1 Tf
1.4683 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F11 1 Tf
0.9921 0 Td
[()4()11()-9()4()6()]TJ
/F7 1 Tf
(-group)Tj
5.1704 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
2.0749 0 Td
(Members)Tj
4.0195 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F11 1 Tf
1.4684 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F11 1 Tf
0.9921 0 Td
[()-3()13(\n)6()4()2()10(\n)6()2()-9()-9()]TJ
/F7 1 Tf
(-group)Tj
7.4437 0 Td
(appear)Tj
2.914 0 Td
(to)Tj
1.0261 0 Td
(feed)Tj
1.8822 0 Td
(exclusively)Tj
4.5128 0 Td
(on)Tj
-34.9793 -1.2983 Td
(toxic)Tj
2.1769 0 Td
(wild)Tj
1.9503 0 Td
(yams)Tj
2.2847 0 Td
(\()Tj
/F11 1 Tf
[()-4()4()7()-3()7()11()20()7()]TJ
/F7 1 Tf
(\))Tj
4.7338 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1)Tj
0 g
(\).)Tj
1.2643 0 Td
(Most)Tj
/F11 1 Tf
2.279 0 Td
[()16()10( )10(\n)6()10()12()-4()]TJ
/F7 1 Tf
3.8324 0 Td
(species)Tj
2.9991 0 Td
(\(~58\))Tj
2.4321 0 Td
(occur)Tj
2.4718 0 Td
(in)Tj
1.0375 0 Td
(habitats)Tj
3.3448 0 Td
(below)Tj
-32.6265 -1.2982 Td
(1,000m)Tj
3.1691 0 Td
(elevation)Tj
3.821 0 Td
(with)Tj
1.9956 0 Td
(few)Tj
1.6158 0 Td
(\(~5\))Tj
1.9559 0 Td
(above)Tj
2.5454 0 Td
(1,500m.)Tj
3.3959 0 Td
(Members)Tj
4.0139 0 Td
(of)Tj
/F11 1 Tf
1.0148 0 Td
[()18(\r)-13()18()6()11( )10(\n)6()10()12()-4()]TJ
/F7 1 Tf
5.6409 0 Td
(are)Tj
1.4513 0 Td
(of)Tj
1.0148 0 Td
(dark)Tj
2.0466 0 Td
(or)Tj
1.0998 0 Td
(cryptic)Tj
-34.7808 -1.3039 Td
(coloration)Tj
4.3313 0 Td
(and)Tj
1.7291 0 Td
(usually)Tj
3.016 0 Td
(occur)Tj
2.4718 0 Td
(above)Tj
2.5455 0 Td
(1,500m.)Tj
/F11 1 Tf
3.3959 0 Td
[()18(\r)-13()18()6()11( )10(\n)6()10()12()-4()]TJ
/F7 1 Tf
5.6352 0 Td
(species)Tj
2.9991 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(subgenus)Tj
/F11 1 Tf
3.9401 0 Td
[()26(\r)-13()17()11()4()11( )10(\n)6()]TJ
-32.5698 -1.2983 Td
[()12()-4()]TJ
/F7 1 Tf
1.3095 0 Td
(\(33)Tj
1.5137 0 Td
(species\))Tj
3.3392 0 Td
(occur)Tj
2.4718 0 Td
(above)Tj
2.5455 0 Td
(2,000m)Tj
3.1691 0 Td
(and)Tj
1.7292 0 Td
(exhibit)Tj
2.9763 0 Td
(epizoic)Tj
3.0387 0 Td
(symbiosis,)Tj
4.3484 0 Td
(i.e.)Tj
1.3606 0 Td
(lichens,)Tj
3.2428 0 Td
(algae)Tj
2.2223 0 Td
(and)Tj
1.7292 0 Td
(moss)Tj
-34.9963 -1.2983 Td
(growing)Tj
3.5262 0 Td
(on)Tj
1.2756 0 Td
(their)Tj
2.109 0 Td
(integument)Tj
4.8415 0 Td
(that)Tj
1.7802 0 Td
(enhance)Tj
3.5489 0 Td
(camouflage)Tj
4.7849 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1)Tj
0 g
(\))Tj
1.0375 0 Td
([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
/F11 1 Tf
3.2541 0 Td
[()18(\r)-13()18()6()11( )10(\n)6()10()12()-4()]TJ
/F7 1 Tf
5.6409 0 Td
(species)Tj
-33.6186 -1.3039 Td
(are)Tj
1.4456 0 Td
(polyphagous,)Tj
5.5105 0 Td
(browsing)Tj
3.9345 0 Td
(on)Tj
1.2756 0 Td
(a)Tj
0.6519 0 Td
(variety)Tj
2.9254 0 Td
(of)Tj
1.0148 0 Td
(plants.)Tj
2.8516 0 Td
(Species)Tj
3.1068 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(third)Tj
2.2166 0 Td
(genus,)Tj
/F11 1 Tf
2.7666 0 Td
[()-4(\n)18()12()6()7()-3()7()12( )10(\n)6()]TJ
/F7 1 Tf
(,)Tj
5.4595 0 Td
(are)Tj
-35.6652 -1.2982 Td
(sometimes)Tj
4.5013 0 Td
(brilliantly)Tj
4.1046 0 Td
(colored)Tj
3.2258 0 Td
(and)Tj
1.7291 0 Td
(other)Tj
2.3471 0 Td
(times)Tj
2.3924 0 Td
(more)Tj
2.3414 0 Td
(cryptic.)Tj
3.2031 0 Td
(Although)Tj
4.0025 0 Td
(their)Tj
2.109 0 Td
(hosts)Tj
2.2734 0 Td
(are)Tj
1.4513 0 Td
(less)Tj
1.6101 0 Td
(well)Tj
-35.2911 -1.2983 Td
(known,)Tj
/F11 1 Tf
3.2314 0 Td
[()-4(\n)18()12()6()7()-3()7()12( )10(\n)6()]TJ
/F7 1 Tf
5.2327 0 Td
(species)Tj
2.9934 0 Td
(can)Tj
1.6271 0 Td
(be)Tj
1.1509 0 Td
(found)Tj
2.6191 0 Td
(at)Tj
0.9525 0 Td
(all)Tj
1.1452 0 Td
(elevational)Tj
4.5014 0 Td
(zones,)Tj
2.6985 0 Td
(with)Tj
2.0013 0 Td
(certain)Tj
2.982 0 Td
(clades)Tj
2.6362 0 Td
(showing)Tj
-33.7717 -1.3039 Td
(some)Tj
2.33 0 Td
(elevational)Tj
4.5014 0 Td
(zonation.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 239.1874 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F0 1 Tf
-6.4961 -1.4976 Td
(UCE)Tj
2.3102 0 Td
(probe)Tj
2.7449 0 Td
(design)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 204.2078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(1,172)Tj
2.3471 0 Td
(UCE)Tj
2.1883 0 Td
(loci)Tj
1.6668 0 Td
(that)Tj
1.7801 0 Td
(we)Tj
1.3266 0 Td
(tested)Tj
2.5512 0 Td
(with)Tj
1.9956 0 Td
(Eupholini)Tj
4.2009 0 Td
(were)Tj
2.1203 0 Td
(identified)Tj
4.0592 0 Td
(in)Tj
1.0374 0 Td
(Faircloth)Tj
3.8211 0 Td
(2017.)Tj
2.3471 0 Td
(The)Tj
-33.2218 -1.2982 Td
(13,674)Tj
2.8232 0 Td
(baits)Tj
2.0863 0 Td
(targeting)Tj
3.7928 0 Td
(these)Tj
2.2507 0 Td
(loci)Tj
1.6667 0 Td
(were)Tj
2.1203 0 Td
(designed)Tj
3.7644 0 Td
(from)Tj
2.2053 0 Td
(six)Tj
1.3153 0 Td
(different)Tj
3.6567 0 Td
(Coleoptera)Tj
4.6091 0 Td
(genomes)Tj
3.77 0 Td
(and)Tj
1.7292 0 Td
(one)Tj
-35.79 -1.3039 Td
(genome)Tj
3.4072 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(sister)Tj
2.3017 0 Td
(Order)Tj
2.6645 0 Td
(Strepsiptera.)Tj
5.1987 0 Td
(Of)Tj
1.2586 0 Td
(these)Tj
2.2507 0 Td
(taxa,)Tj
/F11 1 Tf
2.0806 0 Td
[()-6()2()6()12()20()4()-4()11()11()18()-4()]TJ
5.6749 0 Td
[( )4()11()6()4()2()20()7()-3()]TJ
/F7 1 Tf
(e)Tj
4.6432 0 Td
(\(Curculio-)Tj
-33.1538 -1.2983 Td
(nidae:)Tj
2.6475 0 Td
(Scolytinae\))Tj
4.6374 0 Td
(is)Tj
0.8448 0 Td
(most)Tj
2.2053 0 Td
(closely)Tj
2.8857 0 Td
(related)Tj
2.9423 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(Eupholini)Tj
4.2009 0 Td
(\(Curculionidae:)Tj
6.5196 0 Td
(Entiminae\):)Tj
4.9606 0 Td
(they)Tj
-34.3386 -1.2983 Td
(shared)Tj
2.8629 0 Td
(a)Tj
0.6577 0 Td
(most)Tj
2.2053 0 Td
(recent)Tj
2.7042 0 Td
(common)Tj
3.8495 0 Td
(ancestor)Tj
3.5829 0 Td
(~105)Tj
2.228 0 Td
(Ma)Tj
1.5478 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(].)Tj
1.5987 0 Td
(We)Tj
1.6101 0 Td
(had)Tj
1.7121 0 Td
(the)Tj
1.4683 0 Td
(bait)Tj
1.7291 0 Td
(set)Tj
1.2983 0 Td
(synthesized)Tj
4.8302 0 Td
(by)Tj
-33.8851 -1.3039 Td
(MYcroarray)Tj
5.1079 0 Td
(\(MYcroarray,)Tj
5.6806 0 Td
(Ann)Tj
2.0013 0 Td
(Arbor)Tj
2.6589 0 Td
(MI,)Tj
1.6781 0 Td
(USA\))Tj
2.4548 0 Td
(as)Tj
1.0148 0 Td
(described,)Tj
4.2802 0 Td
(with)Tj
1.9956 0 Td
(no)Tj
1.2756 0 Td
(modifications.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 112.9889 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Laboratory)Tj
4.9417 0 Td
(protocols)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 95.9811 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(We)Tj
1.61 0 Td
(extracted)Tj
3.8835 0 Td
(DNA)Tj
2.3754 0 Td
(non-destructively)Tj
7.251 0 Td
(from)Tj
2.211 0 Td
(48)Tj
1.1678 0 Td
(samples,)Tj
3.6113 0 Td
(using)Tj
2.3868 0 Td
(DNeasy)Tj
3.3675 0 Td
(Tissue)Tj
2.7723 0 Td
(kits)Tj
1.6384 0 Td
(\(Qiagen;)Tj
-32.275 -1.2982 Td
(Macherey-Nagel\).)Tj
7.3927 0 Td
(Nine)Tj
2.194 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2563 0 Td
(48)Tj
1.1679 0 Td
(samples)Tj
3.3845 0 Td
(were)Tj
2.1203 0 Td
(pinned)Tj
3.0558 0 Td
(museum)Tj
3.6963 0 Td
(specimens)Tj
4.3653 0 Td
(that)Tj
1.7802 0 Td
(ranged)Tj
2.9877 0 Td
(in)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 313.6818 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Ultraconser)Tj
5.0244 0 Td
(ved)Tj
1.8 0 Td
(elements)Tj
4.2095 0 Td
(\(UCEs\))Tj
3.4512 0 Td
(resolve)Tj
3.3874 0 Td
(the)Tj
1.5874 0 Td
(phyloge)Tj
3.43 0 Td
(ny)Tj
1.2614 0 Td
(of)Tj
1.0347 0 Td
(the)Tj
1.5874 0 Td
(smurf-weevils)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01880)Tj
4.089 0 Td
(44)Tj
0 g
2.0906 0 Td
(November)Tj
4.7622 0 Td
(22,)Tj
1.5875 0 Td
(2017)Tj
32.5561 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(21)Tj
/F9 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(design,)Tj
2.8984 0 Td
(data)Tj
1.7929 0 Td
(collection)Tj
3.7418 0 Td
(and)Tj
1.5874 0 Td
(analysis,)Tj
3.4087 0 Td
(decision)Tj
3.2953 0 Td
(to)Tj
-16.7245 -1.3748 Td
(publish,)Tj
3.1464 0 Td
(or)Tj
0.9993 0 Td
(preparation)Tj
4.4433 0 Td
(of)Tj
0.9496 0 Td
(the)Tj
1.3677 0 Td
(manuscript.)Tj
/F8 1 Tf
-10.9063 -2.126 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F9 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3749 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
36 0 obj
<>stream
endstream
endobj
37 0 obj
<>stream
endstream
endobj
38 0 obj
<>stream
endstream
endobj
39 0 obj
<>stream
endstream
endobj
40 0 obj
<>stream
endstream
endobj
41 0 obj
<>stream
endstream
endobj
42 0 obj
<>stream
endstream
endobj
43 0 obj
<>stream
endstream
endobj
44 0 obj
<>stream
endstream
endobj
45 0 obj
<>stream
endstream
endobj
46 0 obj
<>stream
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
endstream
endobj
50 0 obj
<>stream
endstream
endobj
51 0 obj
<>stream
endstream
endobj
52 0 obj
<>stream
endstream
endobj
53 0 obj
<>stream
BT
10.3394 79.2147 Td
(exist.)Tj
ET
Q
endstream
endobj
54 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 12 0 R/Annots 57 0 R/Contents 58 0 R/TrimBox[0 0 612 792]>>
endobj
56 0 obj
<>stream
x SƛATB%2HRL>E4gQ2d<TB")
q̜y|˵g}Ͻzg=Zu{]^3f{u]ʿA/O~r z%n9o5sq
|c=LK=馛@`ҤIzOxm
EA)3HD HD HD Hf4ZhW<m9|+j'_~^N۹;\p=
<_ &`=J@"$@"$@"$@"0>;lĽBW\qE^cWyA8po6QP\pzh
7ܰ˾+{(3ĉ{YD HD HD HD qZSWntZxر*?ilV?яJ#}Qd?o#[7뙵JD HD HD H(o*sҳc7aYf!6nkկ~}s=+kw3[Z
;â.?Un^*HD HD HD H: 0
hAY愍7|Jo]p[| 5 !H"o}g׳a[:ȆnN;urK/8L6~?E["M}.>"Mυm{G6z(pEы%XP瞻ΩWe}f42+Ahw32YAɘq+B,H@j6Fx_m+Md'>?p@JU''V3g"$@"$@"$@"fv|˚k9qDChaJ3^Bx!OZ*[n9֘p'$S%AH5X#Co|c\,!
RH-A'dbiع\vG>Qibg"_nq^*1}!q*,[uwKhǏK,ǥP\jfq >
{+nEgj6Lzx)Xbeo