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Milena Schmidt, Stefan Unterer, Jan S. Suchodolski, Julia B. Honneffer, Blake C. Guard, Jonathan A. Lidbury, Jörg M. Steiner, Julia Fritz, Petra Kölle
The fecal microbiome and metabolome differs between dogs fed Bones and Raw Food (BARF) diets and dogs fed commercial diets
www.plosone.org
www.plosone.org
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(35)Tj
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(six)Tj
1.3153 0 Td
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(612)Tj
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3.5036 0 Td
(16)Tj
-36.3172 -1.2983 Td
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1.576 0 Td
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2.0012 0 Td
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4.3994 0 Td
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1.3833 0 Td
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0.9524 0 Td
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(12)Tj
1.1678 0 Td
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3.2825 0 Td
(and)Tj
1.7348 0 Td
(two)Tj
1.7065 0 Td
(dogs)Tj
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1.7121 0 Td
(never)Tj
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(215)Tj
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1 j
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11.9999 0 0 11.9999 200.0125 294.9732 cm
BT
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1.0148 0 Td
(a)Tj
0.652 0 Td
(lyophilized)Tj
-31.1582 -1.2982 Td
(sample,)Tj
3.2484 0 Td
(homogenization)Tj
6.7521 0 Td
(and)Tj
1.7291 0 Td
(extraction)Tj
4.2577 0 Td
(followed.)Tj
3.8721 0 Td
(After)Tj
2.2847 0 Td
(centrifugation,)Tj
6.1171 0 Td
(the)Tj
1.4683 0 Td
(supernatant)Tj
4.9833 0 Td
(was)Tj
-34.7128 -1.2983 Td
(resuspended)Tj
5.2327 0 Td
(in)Tj
1.0318 0 Td
(methanol/chlorofo)Tj
7.5174 0 Td
(rm.)Tj
1.6328 0 Td
(After)Tj
2.2847 0 Td
(adding)Tj
2.9933 0 Td
(internal)Tj
3.3562 0 Td
(standards)Tj
4.0819 0 Td
(derivatization)Tj
5.7316 0 Td
(by)Tj
1.1849 0 Td
(meth-)Tj
-35.0473 -1.3039 Td
(oxyamine)Tj
4.1385 0 Td
(hydrochloride)Tj
5.9017 0 Td
(and)Tj
1.7291 0 Td
(N-methyl-N-trimethy)Tj
8.759 0 Td
(lsilyltrifluoroacetamide)Tj
9.4734 0 Td
(was)Tj
1.7007 0 Td
(used.)Tj
-30.5062 -1.2983 Td
(For)Tj
1.627 0 Td
(detection,)Tj
4.1669 0 Td
(a)Tj
0.652 0 Td
(temperature-gradient)Tj
8.8157 0 Td
(programmed)Tj
5.4765 0 Td
(gas)Tj
1.4797 0 Td
(chromatograph)Tj
6.4232 0 Td
(\(oven)Tj
2.4945 0 Td
(50C)Tj
2.228 0 Td
(to)Tj
-34.5597 -1.2983 Td
(330C)Tj
2.6985 0 Td
(at)Tj
0.9468 0 Td
(20C/min,)Tj
4.4107 0 Td
(injector)Tj
3.3108 0 Td
(50C)Tj
2.2224 0 Td
(to)Tj
1.0204 0 Td
(250C)Tj
2.6986 0 Td
(at)Tj
0.9468 0 Td
(12C/sec\))Tj
4.0988 0 Td
(coupled)Tj
3.3902 0 Td
(with)Tj
1.9899 0 Td
(a)Tj
0.652 0 Td
(helium)Tj
3.0274 0 Td
(carrier)Tj
2.8743 0 Td
(gas)Tj
1.4797 0 Td
(con-)Tj
-35.7673 -1.3039 Td
(taining)Tj
3.05 0 Td
(Leco)Tj
2.109 0 Td
(Pegasus)Tj
3.3505 0 Td
(IV)Tj
1.2586 0 Td
(mass)Tj
2.194 0 Td
(spectrometer)Tj
5.4595 0 Td
(\(scanning)Tj
4.1555 0 Td
(70)Tj
1.1679 0 Td
(spectra/sec)Tj
4.5864 0 Td
(from)Tj
2.2054 0 Td
(80500)Tj
3.1181 0 Td
(Da,)Tj
1.6157 0 Td
(-70)Tj
1.5194 0 Td
(eV)Tj
-35.79 -1.2982 Td
(ionization)Tj
4.2746 0 Td
(energy,)Tj
3.1351 0 Td
(1800)Tj
2.1203 0 Td
(V)Tj
0.9184 0 Td
(detector)Tj
3.4922 0 Td
(voltage\))Tj
3.3846 0 Td
(was)Tj
1.7008 0 Td
(used.)Tj
2.2847 0 Td
(For)Tj
1.6271 0 Td
(detection)Tj
3.9401 0 Td
(0.5)Tj
/F13 1 Tf
1.3946 0 Td
[()]TJ
/F7 1 Tf
(l)Tj
0.9751 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(sample)Tj
3.0217 0 Td
(was)Tj
-34.7525 -1.2983 Td
(injected)Tj
3.3902 0 Td
(onto)Tj
2.0806 0 Td
(a)Tj
0.6519 0 Td
(Restek)Tj
2.846 0 Td
(rtx5Sil-mass)Tj
5.142 0 Td
(spectrometry)Tj
5.4935 0 Td
(column)Tj
3.2882 0 Td
(using)Tj
2.3924 0 Td
(splitness)Tj
3.6057 0 Td
(mode.)Tj
2.7212 0 Td
(To)Tj
1.338 0 Td
(process)Tj
-32.9497 -1.3039 Td
(raw)Tj
1.7064 0 Td
(data)Tj
1.9162 0 Td
(files,)Tj
2.0296 0 Td
(ChromaTOF)Tj
5.4311 0 Td
(v.)Tj
0.8958 0 Td
(2.32)Tj
1.8765 0 Td
(software)Tj
3.583 0 Td
(was)Tj
1.7008 0 Td
(used)Tj
2.0579 0 Td
(and)Tj
1.7348 0 Td
(results)Tj
2.8063 0 Td
(were)Tj
2.1259 0 Td
(uploaded)Tj
3.9288 0 Td
(to)Tj
1.0262 0 Td
(metabolo-)Tj
-32.8193 -1.2983 Td
(micsworkbench.org.)Tj
8.3734 0 Td
(For)Tj
1.6271 0 Td
(further)Tj
3.0274 0 Td
(assessment,)Tj
4.8359 0 Td
(the)Tj
1.4683 0 Td
(data)Tj
1.9162 0 Td
(table)Tj
2.126 0 Td
(was)Tj
1.7008 0 Td
(filtered)Tj
3.067 0 Td
(for)Tj
1.3833 0 Td
(metabolites)Tj
4.8076 0 Td
(of)Tj
-34.333 -1.2982 Td
(unknown)Tj
4.0818 0 Td
(identity)Tj
3.3052 0 Td
(which)Tj
2.6475 0 Td
(were)Tj
2.126 0 Td
(excluded)Tj
3.7814 0 Td
(and)Tj
1.7291 0 Td
(afterward)Tj
4.0535 0 Td
(peak)Tj
2.092 0 Td
(heights)Tj
3.0897 0 Td
(were)Tj
2.126 0 Td
(uploaded)Tj
3.9288 0 Td
(to)Tj
1.0261 0 Td
(Meta-)Tj
-33.9871 -1.2983 Td
(boAnalyst)Tj
4.2576 0 Td
(3.0,)Tj
1.6214 0 Td
(followed)Tj
3.651 0 Td
(by)Tj
1.1848 0 Td
(log)Tj
1.4344 0 Td
(transformation)Tj
6.2758 0 Td
(and)Tj
1.7292 0 Td
(Pareto)Tj
2.8119 0 Td
(scaling.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 347.0173 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Quantitative)Tj
5.5087 0 Td
(real-time)Tj
4.0394 0 Td
(PCR)Tj
2.1449 0 Td
(\(qPCR\))Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 330.0094 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(A)Tj
0.9014 0 Td
(qPCR)Tj
2.5738 0 Td
(was)Tj
1.6951 0 Td
(performed)Tj
4.4731 0 Td
(for)Tj
1.3833 0 Td
(selected)Tj
3.3505 0 Td
(bacterial)Tj
3.6226 0 Td
(groups:)Tj
/F14 1 Tf
3.1975 0 Td
[()31()6()7()7()11()12()14()4()6()-4()11()2( )-9()12(\n)12()]TJ
/F7 1 Tf
6.9335 0 Td
(spp.,)Tj
/F14 1 Tf
2.0636 0 Td
[()25(\n)-4()-3(\r)13()4()6()-4()11()2( )-9()12(\n)12()]TJ
/F7 1 Tf
-30.1944 -1.2982 Td
(spp.,)Tj
/F14 1 Tf
2.0636 0 Td
[()9()4()16(\n)14()7()4()]TJ
/F7 1 Tf
3.0897 0 Td
(spp.,)Tj
/F14 1 Tf
2.0693 0 Td
[()10()7( )20()2()12()11(\r)4()7(\r)4()7()1(\n)-4()]TJ
/F7 1 Tf
5.4028 0 Td
(spp.,)Tj
/F14 1 Tf
2.0636 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F14 1 Tf
0.9978 0 Td
[()7(\r)10()12()]TJ
/F7 1 Tf
(,)Tj
/F14 1 Tf
1.7801 0 Td
[()1()4(\r)7()9()7( )-9()4()12()12(\n)12()]TJ
4.8699 0 Td
[()18()12( )20()16()6(\r)11()18()-4()]TJ
/F7 1 Tf
(,)Tj
/F14 1 Tf
4.1839 0 Td
[()75(\n)12( )-9()4()1()14()4()6()-4()11()2( )]TJ
/F7 1 Tf
(,)Tj
5.2157 0 Td
(and)Tj
/F14 1 Tf
1.7292 0 Td
[()-2()16()15()12()3()4(\r)]TJ
-33.4656 -1.3039 Td
[()4()6()-4()11()2( )]TJ
/F7 1 Tf
2.6021 0 Td
(spp.)Tj
1.8425 0 Td
(A)Tj
0.9015 0 Td
(CFX)Tj
2.0636 0 Td
(96)Tj
1.1735 0 Td
(Touch)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 314.022 308.4661 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(TM)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 327.4582 303.9874 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Real-Time)Tj
4.4163 0 Td
(PCR)Tj
2.0693 0 Td
(Detection)Tj
4.1442 0 Td
(system)Tj
2.9367 0 Td
(\(Biorad)Tj
3.2485 0 Td
(Laboratories\))Tj
5.5785 0 Td
(was)Tj
-35.1379 -1.2982 Td
(used)Tj
2.0579 0 Td
(for)Tj
1.389 0 Td
(examination)Tj
5.2327 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(samples.)Tj
3.6056 0 Td
(As)Tj
1.2642 0 Td
(described)Tj
4.0535 0 Td
(previously)Tj
4.354 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(],)Tj
3.5433 0 Td
(10)Tj
/F13 1 Tf
1.1735 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2643 0 Td
(TaqMan)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 538.4692 295.4267 cm
BT
/F12 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 548.1637 291.0047 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(reac-)Tj
-34.8148 -1.2982 Td
(tion)Tj
1.8425 0 Td
(mixtures)Tj
3.753 0 Td
(\(5)Tj
/F13 1 Tf
1.0318 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2643 0 Td
(of)Tj
1.0148 0 Td
(TaqMan)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 323.3763 282.444 cm
BT
/F12 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 333.0708 278.022 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Fast)Tj
1.8368 0 Td
(Universal)Tj
4.0648 0 Td
(PCR)Tj
2.0637 0 Td
(master)Tj
2.9253 0 Td
(mix)Tj
1.7745 0 Td
(\(2)Tj
/F13 1 Tf
[()]TJ
/F7 1 Tf
(\),)Tj
2.1713 0 Td
(No)Tj
1.4627 0 Td
(AmpErase)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 537.8456 282.444 cm
BT
/F12 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 547.5401 278.022 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(UNG)Tj
-34.7525 -1.3039 Td
(\(Applied)Tj
3.7417 0 Td
(Biosystems\),)Tj
5.2214 0 Td
(1)Tj
/F13 1 Tf
0.6917 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2642 0 Td
(of)Tj
1.0148 0 Td
(water,)Tj
2.6589 0 Td
(0.4)Tj
/F13 1 Tf
1.3946 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2699 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0239 0 Td
(primer)Tj
2.9934 0 Td
(\(final)Tj
2.3414 0 Td
(concentration:)Tj
6.0434 0 Td
(400)Tj
1.6498 0 Td
(nM\),)Tj
-33.3239 -1.2982 Td
(0.2)Tj
/F13 1 Tf
1.3946 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2643 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(probe)Tj
2.5455 0 Td
(\(final)Tj
2.3414 0 Td
(concentration:)Tj
6.0434 0 Td
(200)Tj
1.6498 0 Td
(nM\),)Tj
2.2223 0 Td
(1)Tj
/F13 1 Tf
0.6973 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2643 0 Td
(of)Tj
1.0148 0 Td
(1%)Tj
1.457 0 Td
(bovine)Tj
2.9253 0 Td
(serum)Tj
2.7213 0 Td
(albumin)Tj
3.5716 0 Td
(\(BSA,)Tj
-33.6017 -1.2983 Td
(final)Tj
2.0013 0 Td
(concentration:)Tj
6.0434 0 Td
(0.1%\),)Tj
2.7212 0 Td
(and)Tj
1.7292 0 Td
(2)Tj
/F13 1 Tf
0.6973 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2642 0 Td
(of)Tj
1.0148 0 Td
(DNA)Tj
2.3754 0 Td
(\(1)Tj
1.0375 0 Td
(:)Tj
0.4479 0 Td
(10)Tj
1.1678 0 Td
(or)Tj
1.0999 0 Td
(1)Tj
0.6973 0 Td
(:)Tj
0.4422 0 Td
(100)Tj
1.6498 0 Td
(dilution\)\))Tj
4.0875 0 Td
(was)Tj
1.7008 0 Td
(used)Tj
2.0579 0 Td
(for)Tj
1.389 0 Td
(further)Tj
-33.6244 -1.3039 Td
(examination,)Tj
5.4595 0 Td
(following)Tj
3.9742 0 Td
(a)Tj
0.6576 0 Td
(protocol)Tj
3.5943 0 Td
(of)Tj
1.0148 0 Td
(95C)Tj
2.228 0 Td
(for)Tj
1.389 0 Td
(20)Tj
1.1678 0 Td
(s,)Tj
0.8051 0 Td
(and)Tj
1.7348 0 Td
(40)Tj
1.1678 0 Td
(cycles)Tj
2.5512 0 Td
(at)Tj
0.9524 0 Td
(95C)Tj
2.2281 0 Td
(for)Tj
1.3889 0 Td
(5)Tj
0.6917 0 Td
(s,)Tj
0.8107 0 Td
(and)Tj
1.7291 0 Td
(10)Tj
1.1679 0 Td
(s)Tj
0.5838 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
-36.2491 -1.2983 Td
(optimized)Tj
4.2576 0 Td
(annealing)Tj
4.1386 0 Td
(temperature.)Tj
5.3518 0 Td
(Ten)Tj
/F13 1 Tf
1.8028 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2642 0 Td
(SYBR-based)Tj
5.1364 0 Td
(reaction)Tj
3.4922 0 Td
(mixtures)Tj
3.7531 0 Td
(\(5)Tj
/F13 1 Tf
1.0375 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2642 0 Td
(of)Tj
1.0148 0 Td
(SsoFast)Tj
/F13 1 Tf
[()]TJ
/F7 1 Tf
-32.5132 -1.2982 Td
(EvaGreen)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 239.4141 204.4346 cm
BT
/F12 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 249.0519 200.0125 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(supermix)Tj
3.9741 0 Td
(\(Biorad)Tj
3.2485 0 Td
(Laboratories\),)Tj
5.8053 0 Td
(1.6)Tj
/F13 1 Tf
1.3946 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2643 0 Td
(of)Tj
1.0148 0 Td
(water,)Tj
2.6589 0 Td
(0.4)Tj
/F13 1 Tf
1.3946 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2642 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(primer)Tj
2.9934 0 Td
(\(final)Tj
2.3414 0 Td
(con-)Tj
-35.3024 -1.3039 Td
(centration:)Tj
4.5638 0 Td
(400)Tj
1.6496 0 Td
(nM\),)Tj
2.2224 0 Td
(1)Tj
/F13 1 Tf
0.6973 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2643 0 Td
(of)Tj
1.0148 0 Td
(1%)Tj
1.4513 0 Td
(BSA)Tj
1.9615 0 Td
(\(final)Tj
2.3471 0 Td
(concentration:)Tj
6.0434 0 Td
(0.1%\),)Tj
2.7156 0 Td
(and)Tj
1.7348 0 Td
(2)Tj
/F13 1 Tf
0.6917 0 Td
[()]TJ
/F7 1 Tf
(L)Tj
1.2642 0 Td
(of)Tj
1.0148 0 Td
(DNA)Tj
2.3811 0 Td
(\(1)Tj
1.0318 0 Td
(:)Tj
0.4479 0 Td
(10)Tj
1.1735 0 Td
(or)Tj
-35.6709 -1.2982 Td
(1)Tj
0.6917 0 Td
(:)Tj
0.4479 0 Td
(100)Tj
1.6497 0 Td
(dilution\)\))Tj
4.0875 0 Td
(were)Tj
2.1203 0 Td
(used)Tj
2.0636 0 Td
(for)Tj
1.3833 0 Td
(a)Tj
0.6576 0 Td
(protocol)Tj
3.5943 0 Td
(of)Tj
1.0148 0 Td
(95C)Tj
2.228 0 Td
(for)Tj
1.3833 0 Td
(2)Tj
0.6973 0 Td
(min,)Tj
2.0806 0 Td
(and)Tj
1.7292 0 Td
(40)Tj
1.1735 0 Td
(cycles)Tj
2.5512 0 Td
(at)Tj
0.9524 0 Td
(95C)Tj
2.228 0 Td
(5)Tj
0.6973 0 Td
(s)Tj
0.5783 0 Td
(and)Tj
1.7291 0 Td
(10)Tj
1.1736 0 Td
(s)Tj
-36.9125 -1.2983 Td
(at)Tj
0.9525 0 Td
(the)Tj
1.4683 0 Td
(optimized)Tj
4.2632 0 Td
(annealing)Tj
4.1329 0 Td
(temperature.)Tj
5.3518 0 Td
(Afterwards)Tj
4.6658 0 Td
(a)Tj
0.6519 0 Td
(melt)Tj
2.0069 0 Td
(curve)Tj
2.4265 0 Td
(analysis)Tj
3.3222 0 Td
(was)Tj
1.7007 0 Td
(set)Tj
1.304 0 Td
(up.)Tj
/F14 1 Tf
1.491 0 Td
[()1()4(\r)7()9()7( )-9()4()]TJ
-33.7377 -1.2983 Td
[()12(\n)12()]TJ
1.7915 0 Td
[()4()2( )-23()-8( )-9()12()13()2()2()]TJ
/F7 1 Tf
4.5353 0 Td
(was)Tj
1.6951 0 Td
(analyzed)Tj
3.7021 0 Td
(using)Tj
2.3924 0 Td
(a)Tj
0.652 0 Td
(probe)Tj
2.5455 0 Td
(based)Tj
2.4774 0 Td
(protocol)Tj
3.5943 0 Td
(as)Tj
1.0148 0 Td
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