Gratzl, Manfred; Dahl, G.
Ca2+-induced fusion of Golgi-derived secretory vesicles isolated from rat liver.
In: FEBS Letters, Vol. 62: S. 142-145
During the transport of plasma proteins from the
cytoplasma of hepatocytes to the extracellular fluid
srnall vesicles may act as shuttles between the Golgi
complex and the plasma membrane. This type of
intracellular transfer is weil established for various
secretory cells and may be adopted also for the
hepatocyte. Recent investigations have shown that
secretory vesicles fuse with each other during
secretion in mast cells  exocrine [5,6] and
endocrine pancreatic tissue . The intervesicular
fusion provides a tool for studies on membrane fusion,
since Golgi-derived vesicles can be isolated from the
hepatocyte and their interaction with various agents,
suggested to trigger membrane fusion, can be
monitored by freeze-cleaving.