Tynan, Katherine and Olsen, Anne and Trask, Barbara and Jong, Pieter de and Thompson, John A. and Zimmermann, Wolfgang and Carrano, Anthony and Mohrenweiser, Harvey
Assembly and Analysis of Cosmid Contigs in the CEA-gene Family Region of Human Chromosome 19.
In: Nucleic Acids Research, Vol. 20, No. 7: pp. 1629-1636
The carcinoembryonic antigen (CEA)-like genes are members of a large gene family which Is part of the immunoglobulln superfamily. The CEA family is divided into two major subgroups, the CEA-subgroup and the pregnancy-specific glycoprotein (PSG)-subgroup. In the course of an effort to develop a set of overlapping cosmlds spanning human chromosome 19, we identified 245 cosmids In a human chromosome 19 cosmid library (6 – 7X redundant) by hybridization with an IgC-like domain fragment of the CEA gene. A fluorescence-based restriction enzyme digest fingerprinting strategy was used to assemble 212 probe-positive cosmids, along with 115 additional cosmids from a collection of ~ 8, 000 randomly selected cosmids, into five contigs. Two of the contigs contain CEA-subgroup genes while the remaining three contigs contain PSG-subgroup genes. These five contigs range in size from 100 kb to over 300 kb and span an estimated 1 Mb. The CEA-like gene family was determined by fluorescence in situ hybridization to map in the q13.1-q13.2 region of human chromosome 19. Analysis of the two CEA-subgroup contigs provided verification of the contig assembly strategy and insight into the organization of 9 CEA-subgroup genes.