Pulsed Interleaved MINFLUX

Masullo, Luciano A.; Steiner, Florian; Zahringer, Jonas; Lopez, Lucia F.; Bohlen, Johann; Richter, Lars; Cole, Fiona; Tinnefeld, Philip and Stefani, Fernando D. (2021): Pulsed Interleaved MINFLUX. In: Nano Letters, Vol. 21, No. 1: pp. 840-846

Abstract

We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.

• Item Type: Journal article
• Faculties: Chemistry and Pharmacy > Department of Chemistry
• Subjects: 500 Science > 540 Chemistry
• ISSN: 1530-6984
• Language: English