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Rettenmaier, Regina; Thieme, Nils; Streubel, Johanna; Di Bello, Luca; Kowollik, Marie-Louise; Huang, Liren; Maus, Irena; Klingl, Andreas; Liebl, Wolfgang and Zverlov, Vladimir V. (2021): Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize- fed biogas fermenter. In: International Journal of Systematic and Evolutionary Microbiology, Vol. 71, No. 11, 5044

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Strain MD1(T) is an anaerobic, Gram-stain-negative bacterium isolated from a lab-scale biogas fermenter fed with maize silage. It has a rod-shaped morphology with peritrichously arranged appendages and forms long chains of cells and coccoid structures. The colonies of MD1(T) were white, circular, slightly convex and had a smooth rim. The isolate is mesophilic, displaying growth between 25 and 45 degrees C with an optimum at 40 degrees C. It grew at pH values of pH 6.7-8.2 (optimum, pH 7.1) and tolerated the addition of up to 1.5% (w/v) NaCl to the medium. The main cellular fatty acids of MD1(T) are C-14:0 DMA and C-16:0. Strain MD1(T) fermented xylose, arabinose, glucose, galactose, cellobiose, maltose, maltodextrin10, lactose starch, and xylan, producing mainly 2-propanol and acetic acid. The genome of the organism has a total length of 4163427 bp with a G+C content of 38.5 mol%. The two closest relatives to MD1(T) are Mobilitalea sibirica P3M-3(T) and Anaerotaenia torta FH052(T) with 96.44 or 95.8 % 16S rRNA gene sequence similarity and POCP values of 46.58 and 50.58%, respectively. As MD1(T) showed saccharolytic and xylanolytic properties, it may play an important role in the biogas fermentation process. Closely related variants of MD1(T) were also abundant in microbial communities involved in methanogenic fermentation. Based on morphological, phylogenetic and genomic data, the isolated strain can be considered as representing a novel genus in the family Lachnospiraceae, for which the name Variimorphobacter saccharofermentans gen. nov., sp. nov. (type strain MD1(T)=DSM 110715(T)=JCM 39125(T)) is proposed.

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