Insights into Regulation of C-2 and C-4 Photosynthesis in Amaranthaceae/Chenopodiaceae Using RNA-Seq

Siadjeu, Christian; Lauterbach, Maximilian und Kadereit, Gudrun (2021): Insights into Regulation of C-2 and C-4 Photosynthesis in Amaranthaceae/Chenopodiaceae Using RNA-Seq. In: International Journal of Molecular Sciences, Bd. 22, Nr. 22, 12120

Abstract

Amaranthaceae (incl. Chenopodiaceae) shows an immense diversity of C-4 syndromes. More than 15 independent origins of C-4 photosynthesis, and the largest number of C-4 species in eudicots signify the importance of this angiosperm lineage in C-4 evolution. Here, we conduct RNA-Seq followed by comparative transcriptome analysis of three species from Camphorosmeae representing related clades with different photosynthetic types: Threlkeldia diffusa (C-3), Sedobassia sedoides (C-2), and Bassia prostrata (C-4). Results show that B. prostrata belongs to the NADP-ME type and core genes encoding for C-4 cycle are significantly upregulated when compared with Sed. sedoides and T. diffusa. Sedobassia sedoides and B. prostrata share a number of upregulated C-4-related genes;however, two C-4 transporters (DIT and TPT) are found significantly upregulated only in Sed. sedoides. Combined analysis of transcription factors (TFs) of the closely related lineages (Camphorosmeae and Salsoleae) revealed that no C-3-specific TFs are higher in C-2 species compared with C-4 species;instead, the C-2 species show their own set of upregulated TFs. Taken together, our study indicates that the hypothesis of the C-2 photosynthesis as a proxy towards C-4 photosynthesis is questionable in Sed. sedoides and more in favour of an independent evolutionary stable state.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Biologie > Department Biologie I
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• Sprache: Englisch