Super-resolution Imaging of Energy Transfer by Intensity-Based STED-FRET

Szalai, Alan M.; Siarry, Bruno; Lukin, Jeronimo; Giusti, Sebastian; Unsain, Nicolas; Caceres, Alfredo; Steiner, Florian; Tinnefeld, Philip; Refojo, Damian; Jovin, Thomas M. and Stefani, Fernando D. (2021): Super-resolution Imaging of Energy Transfer by Intensity-Based STED-FRET. In: Nano Letters, Vol. 21, No. 5: pp. 2296-2303

Abstract

Forster resonance energy transfer (FRET) imaging methods provide unique insight into the spatial distribution of energy transfer and (bio)molecular interaction events, though they deliver average information for an ensemble of events included in a diffraction-limited volume. Coupling super-resolution fluorescence microscopy and FRET has been a challenging and elusive task. Here, we present STED-FRET, a method of general applicability to obtain super-resolved energy transfer images. In addition to higher spatial resolution, STED-FRET provides a more accurate quantification of interaction and has the capacity of suppressing contributions of noninteracting partners, which are otherwise masked by averaging in conventional imaging. The method capabilities were first demonstrated on DNA-origami model systems, verified on uniformly double-labeled microtubules, and then utilized to image biomolecular interactions in the membrane-associated periodic skeleton (MPS) of neurons.

• Item Type: Journal article
• Faculties: Chemistry and Pharmacy > Department of Chemistry
• Subjects: 500 Science > 540 Chemistry
• ISSN: 1530-6984
• Language: English