HutT functions as the major L-histidine transporter in Pseudomonas putida KT2440

Wirtz, Larissa; Eder, Michelle; Brand, Anna-Katharina und Jung, Heinrich (2021): HutT functions as the major L-histidine transporter in Pseudomonas putida KT2440. In: Febs Letters, Bd. 595, Nr. 16: S. 2113-2126

Abstract

Histidine is an important carbon and nitrogen source of gamma-proteobacteria and can affect bacteria-host interactions. The mechanisms of histidine uptake are only partly understood. Here, we analyze functional properties of the putative histidine transporter HutT of the soil bacterium Pseudomonas putida. The hutT gene is part of the histidine utilization operon, and the gene product belongs to the amino acid-polyamine-organocation (APC) family of secondary transporters. Deletion of hutT severely impairs growth of P. putida on histidine, suggesting that the encoded transporter is the major histidine uptake system of P. putida. Transport experiments with cells and purified and reconstituted protein indicate that HutT functions as a high-affinity histidine : proton symporter with high specificity for the amino acid. Substitution analyses identified amino acids crucial for HutT function.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Biologie > Department Biologie I
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• ISSN: 0014-5793
• Sprache: Englisch