Increasing transparency and reproducibility in stroke-microbiota research: A toolbox for microbiota analysis

Sorbie, Adam; Delgado Jiménez, Rosa and Benakis, Corinne (24. February 2022): Increasing transparency and reproducibility in stroke-microbiota research: A toolbox for microbiota analysis. In: iScience

Abstract

Homeostasis of gut microbiota is crucial in maintaining human health. Alterations, or “dysbiosis”, are increasingly implicated in human diseases, such as cancer, inflammatory bowel diseases and more recently neurological disorders. In ischemic stroke patients, gut microbial profiles are markedly different compared to healthy controls, while manipulation of microbiota in animal models of stroke, modulates outcome, further implicating microbiota in stroke pathobiology. Despite this, evidence for the involvement of specific microbes or microbial products and microbial signatures have yet to be identified, likely due to differences in methodology, data analysis and confounding variables between different studies. Here, we provide a set of guidelines to enable researchers to conduct high quality, reproducible and transparent microbiota studies, focusing on 16S rRNA sequencing in the emerging subfield of the stroke-microbiota. In doing so, we aim to facilitate novel and reproducible associations between the microbiota and brain diseases including stroke, and translation into clinical practice.

• Item Type: Journal article
• Faculties: Medicine > Institute for Stroke and Dementia Research (ISD)
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 2589-0042
• Language: English
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892