Specific Localization and Quantification of the Oligo‐Mouse‐Microbiota (OMM 12 ) by Fluorescence In Situ Hybridization (FISH)

Brugiroux, Sandrine; Berry, David; Ring, Diana; Barnich, Nicolas; Daims, Holger und Stecher, Bärbel (2022): Specific Localization and Quantification of the Oligo‐Mouse‐Microbiota (OMM 12 ) by Fluorescence In Situ Hybridization (FISH). In: Current Protocols, Bd. 2, Nr. 9

Abstract

The oligo-mouse-microbiota (OMM12) is a widely used syncom that colonizes gnotobiotic mice in a stable manner. It provides several fundamental functions to its murine host, including colonization resistance against enteric pathogens. Here, we designed and validated specific fluorescence in situ hybridization (FISH) probes to detect and quantify OMM12 strains on intestinal tissue cross sections. 16S rRNA‒specific probes were designed, and specificity was validated on fixed pure cultures. A hybridization protocol was optimized for sensitive detection of the individual bacterial cells in cryosections. Using this method, we showed that the intestinal mucosal niche of Akkermansia muciniphila can be influenced by global gut microbial community context. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin > Max-von-Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 2691-1299
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892