Effects of matcha tea extract on cell viability and peroxisome proliferator-activated receptor gamma expression on T47D breast cancer cells

Keckstein, Simon; Tilgener, Constantin; Jeschke, Udo ; Hofmann, Simone; Vilsmaier, Theresa; Kaltofen, Till; Heidegger, Helene; Batz, Falk; Mahner, Sven und Schröder, Lennard (2022): Effects of matcha tea extract on cell viability and peroxisome proliferator-activated receptor gamma expression on T47D breast cancer cells. In: Archives of Gynecology and Obstetrics, Bd. 306, Nr. 2: S. 451-459

Abstract

Purpose In the following work, we investigated the nuclear peroxisome proliferator-activated receptor gamma (PPAR gamma)dependent proliferation behavior of breast cancer cells after stimulation with matcha green tea extract (MTE). Methods T47D cells were stimulated with MTE at concentrations of 5, 10 and 50 mg/ml. Cell viability was assessed using a WST-1 assay after an incubation time of 72 h. PPAR gamma expression was quantified at the gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in T47D cells after 72 h at 5, 10 and 50 mg/ml. The PCR showed an overexpression of PPAR gamma in T47D cells in all concentrations. At the concentration of 50 mu g/ml the expression was significantly increased (p < 0.05). The WB demonstrated a significant quantitative increase of PPAR gamma at protein level with MTE concentrations of 10 and 50 mg/ml. In addition, there was a negative correlation between the overexpression of PPAR gamma and the inhibition of proliferation. Conclusion MTE decreases the cell viability of T47D cells and furthermore leads to an overexpression of PPAR gamma on protein and mRNA level.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0932-0067
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892