Detection of apoptosis by [F-18]ML-10 after cardiac ischemia-reperfusion injury in mice

Fischer, Maximilian; Zacherl, Mathias J.; Olivier, Jessica; Lindner, Simon; Massberg, Steffen; Bartenstein, Peter; Grawe, Freba; Ziegler, Sibylle; Brendel, Matthias; Lehner, Sebastian; Böning, Guido und Todica, Andrei (2022): Detection of apoptosis by [F-18]ML-10 after cardiac ischemia-reperfusion injury in mice. In: Annals of Nuclear Medicine, Bd. 37, Nr. 1: S. 34-43

Abstract

Objective Myocardial infarction leads to ischemic heart disease and cell death, which is still a major obstacle in western society. In vivo imaging of apoptosis, a defined cascade of cell death, could identify myocardial tissue at risk. Methods Using 2-(5-[F-18]fluoropentyl)-2-methyl-malonic acid ([F-18]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in a mouse model of transient ligation of the left anterior descending (LAD) artery. 2-deoxy-2-[18F]fluoro-D-glucose ([F-18]FDG) PET imaging indicated the defect area. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) histology stain indicated cardiac apoptosis. Results [F-18]ML-10 uptake was evident in the ischemic area after transient LAD ligation in ex vivo autoradiography and in vivo PET imaging. Detection of [F-18]ML-10 is in line with the defect visualized by [F-18]FDG and the histological approach of TUNEL staining. Conclusion The tracer [F-18]ML-10 is suitable for detecting apoptosis after transient LAD ligation in mice.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0914-7187
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892