Third‐Generation Sequencing of Epigenetic DNA

Searle, Bethany; Müller, Markus ; Carell, Thomas und Kellett, Andrew (27. March 2023): Third‐Generation Sequencing of Epigenetic DNA. In: Angewandte Chemie International Edition, Vol. 62, No. 14, e202215704

Abstract

The discovery of epigenetic bases has revolutionised the understanding of disease and development. Among the most studied epigenetic marks are cytosines covalently modified at the 5 position. In order to gain insight into their biological significance, the ability to determine their spatiotemporal distribution within the genome is essential. Techniques for sequencing on “next-generation” platforms often involve harsh chemical treatments leading to sample degradation. Third-generation sequencing promises to further revolutionise the field by providing long reads, enabling coverage of highly repetitive regions of the genome or structural variants considered unmappable by next generation sequencing technology. While the ability of third-generation platforms to directly detect epigenetic modifications is continuously improving, at present chemical or enzymatic derivatisation presents the most convenient means of enhancing reliability. This Review presents techniques available for the detection of cytosine modifications on third-generation platforms.

• Item Type: Journal article
• EU Funded Grant Agreement Number: 861381
• EU Projects: Horizon 2020 > Marie Skłodowska Curie Actions > Marie Skłodowska Curie: Fostering new skills by means of excellent initial training of researchers > 861381: NATURE-ETN - Nucleic Acids for Future Gene Editing, Immunotherapy and Epigenetic Sequence Modification
• Form of publication: Publisher's Version
• Faculties: Chemistry and Pharmacy > Department of Chemistry
• Subjects: 500 Science > 540 Chemistry
• Language: English