ORCID: https://orcid.org/0000-0002-3156-2976; Biere, Katharina; Liemersdorf, Christian; Tuschen, Marina; Hemmersbach, Ruth and Chouker, Alexander
ORCID: https://orcid.org/0000-0002-0133-4104
(2023):
Differential effects of hypergravity on immune dysfunctions induced by simulated microgravity.
In: The FASEB Journal, Vol. 37, No. 5
[PDF, 326kB]
Abstract
Microgravity (μg) is among the major stressors in space causing immune cell dysregulations. These are frequently expressed as increased pro-inflammatory states of monocytes and reduced activation capacities in T cells. Hypergravity (as artificial gravity) has shown to have beneficial effects on the musculoskeletal and cardiovascular system both as a countermeasure option for μg-related deconditioning and as “gravitational therapy” on Earth. Since the impact of hypergravity on immune cells is sparsely explored, we investigated if an application of “mild” mechanical loading of 2.8 g is able to avoid or treat μg-mediated immune dysregulations. For this, T cell and monocyte activation states and cytokine pattern were first analyzed after whole blood antigen incubation in simulated μg (s-μg) by using the principle of fast clinorotation or in hypergravity. Subsequent hypergravity countermeasure approaches were run at three different sequences: one preconditioning setting, where 2.8 g was applied before s-μg exposure and two therapeutic approaches in which 2.8 g was set either intermediately or at the end of s-μg. In single g-grade exposure experiments, monocyte pro-inflammatory state was enhanced in s-μg and reduced in hypergravity, whereas T cells displayed reduced activation when antigen incubation was performed in s-μg. Hypergravity application in all three sequences did not alleviate the increased pro-inflammatory potential of monocytes. However, in T cells the preconditioning approach restored antigen-induced CD69 expression and IFNγ secretion to 1 g control values and beyond. This in vitro study demonstrates a proof of concept that mild hypergravity is a gravitational preconditioning option to avoid adaptive immune cell dysfunctions induced by (s-)μg and that it may act as a booster of immune cell functions.
Item Type: | Journal article |
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Faculties: | Medicine > Medical Center of the University of Munich > Clinic for Anaesthesiology |
Subjects: | 600 Technology > 610 Medicine and health |
URN: | urn:nbn:de:bvb:19-epub-108870-6 |
ISSN: | 0892-6638 |
Language: | English |
Item ID: | 108870 |
Date Deposited: | 22. Mar 2024, 07:24 |
Last Modified: | 22. Mar 2024, 07:24 |