Disrupted biomineralization in zebra mussels after exposure to bisphenol-A: Potential implications for molar-incisor hypomineralization

Liu, Fangfang; Reichl, Franz-Xaver; Milz, Stefan; Woelfle, Uta Christine; Kuehnisch, Jan; Schmitz, Christoph; Geist, Jürgen; Hickel, Reinhard; Hoegg, Christof und Sternecker, Katharina (2022): Disrupted biomineralization in zebra mussels after exposure to bisphenol-A: Potential implications for molar-incisor hypomineralization. In: Dental Materials, Bd. 38, Nr. 4: S. 689-699

Abstract

Objective: The aetiology of molar-incisor hypomineralization (MIH) is currently unclear. A major hurdle in MIH research is the lack of adequate model systems. The study investigated the feasibility of zebra mussel (Dreissena polymorpha) as a novel model to screen potential MIH-related factors. Methods: In four experiments with overall 46 groups (n = 7 mussels/group), six groups per experiment were incubated with 100 mg/l calcein (mineralization marker) solution for 96 h to evaluate the dynamics of shell biomineralization, another six groups with tap water only (controls). Then zebra mussels with and without calcein pre-incubation were exposed to cadmium sulfate hydrate (3CdSO(4)center dot 8H(2)O) (positive control;0, 0.01, 0.1, 1, 10 and 100 mg/l), possible aetiological factors of MIH including bisphenol-A (BPA;0, 0.02, 0.2, 2, 20 and 200 mg/l) and erythromycin (0, 0.1, 1, 10, 100 and 1000 mg/l) as mineralization disruptors , and doxycycline (0, 0.1, 1, 10, 100 and 1000 mg/l) for 96 h, respectively. After two weeks, the mussels were sacrificed and shells were embedded in methylmethacrylate for fluorescence intensity analysis. Results: Mortality rate was 100% after 20, 200 mg/l BPA and 10, 100 mg/l 3CdSO(4)center dot 8H(2)O exposure. Thereby, the median lethal concentration (96 h-LC50) of BPA was 6.3 mg/l (95% CI, 1.3-34.4 mg/l), and that of cadmium was 3.1 mg/l (95% CI, 0.7-10.5 mg/l). Notably, calcein fluorescence in shells significantly decreased (p < 0.05) after 2 mg/l BPA and 1 mg/l 3CdSO4 center dot 8H2O exposure. Significance: These findings suggest that BPA may disrupt biomineralization. Biomineralization in zebra mussels seems to be an effective model for investigating po-tential MIH-related factors. (c) 2022 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0109-5641
• Sprache: Englisch