Leukemia derived dendritic cell (DCleu) mediated immune response goes along with reduced (leukemia-specific) regulatory T-cells

Pepeldjiyska, Elena; Li, Lin; Gao, Jincheng; Seidel, Corinna L.; Blasi, Christian; Oezkaya, Erdem; Schmohl, Jörg; Kraemer, Doris; Schmid, Christoph; Rank, Andreas and Schmetzer, Helga Maria (2022): Leukemia derived dendritic cell (DCleu) mediated immune response goes along with reduced (leukemia-specific) regulatory T-cells. In: Immunobiology, Vol. 227, No. 4, 152237

Abstract

The blastmodulatory Kit-M, composed of granulocyte-macrophage colony-stimulating-factor (GM-CSF) and Prostaglandin E1 (PGE(1)), is known to convert myeloid leukaemic blasts (from AML patients) into leukaemia derived dendritic cells (DCleu), which activate immunoreactive cells to gain antileukemic/leukaemia-specific activity. In this study we had a special focus on the influence of Kit-M treated, DC/DCleu containing patient-s'whole blood (WB, n = 16) on the provision of immunosuppressive regulatory T-cells. We could confirm that Kit-M significantly increased frequencies of (mature) dendritic cells (DC) and DCleu from leukemic whole blood (WB) without induction of blast proliferation. After mixed lymphocyte culture (MLC) with patients' T-cells we confirmed that DCleu mediated leukemia-specific responses-going along with activated and leukemia-specific T-and NK-cells in an intracellular cytokine staining assay (ICS) and a degranulation assay (Deg)-resulted in an increased anti-leukemic cytotoxicity (Cytotoxicity Fluorolysis Assay = CTX). We could demonstrate that (leukemia-specific) CD4(+ )and CD8(+) regulatory T-cell population (T-reg) decreased significantly

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 0171-2985
• Language: English