An improved imaging system that corrects MS2-induced RNA destabilization

Li, Weihan; Maekiniemi, Anna; Sato, Hanae; Osman, Christof und Singer, Robert H. (2022): An improved imaging system that corrects MS2-induced RNA destabilization. In: Nature Methods, Bd. 19, Nr. 12: S. 1558-1562

Abstract

An improved version of the MS2-MCP system for imaging RNA dynamics involves tethering translation termination factors to tagged mRNAs to bypass destabilization caused by NMD machinery. The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Biologie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• ISSN: 1548-7091
• Sprache: Englisch