Multi-Scale Investigation of Human Renal Tissue in Three Dimensions

Busse, M.; Ferstl, S.; Kimm, M. A.; Hehn, L.; Steiger, K.; Allner, S.; Mueller, M.; Drecoll, E.; Buerkner, T.; Dierolf, M.; Gleich, B.; Weichert, W. und Pfeiffer, F. (2022): Multi-Scale Investigation of Human Renal Tissue in Three Dimensions. In: IEEE Transactions on Medical Imaging, Bd. 41, Nr. 12: S. 3489-3497

Abstract

Histopathology as a diagnostic mainstay for tissue evaluation is strictly a 2D technology. Combining and supplementing this technology with 3D imaging has been proposed as one future avenue towards refining comprehensive tissue analysis. To this end, we have developed a laboratory-based X-ray method allowing for the investigation of tissue samples in three dimensions with isotropic volume information. To assess the potential of our method for micro- morphology evaluation, we selected several kidneyregions fromthreepatientswith cystic kidney disease, obstructive nephropathy and diabetic glomerulopathy. Tissue specimens were processed using our in-house-developed X-ray eosin stain and investigated with a commercial microCT and our in- house-built NanoCT. The microCT system provided overview scans with voxel sizes of 14.8 mu m and the NanoCT was employed for higher resolutions including voxel sizes from 1.4 mu m to 210 nm. We present a methodology allowing for a precise micromorphologic investigation in three dimensions which is compatible with conventional histology. Advantages of our methodology are its versatility with respect to multi-scale investigations, being laboratory-based, allowing for nondestructive imaging and providing isotropic volume information. We believe, that after future developmentalwork this method might contribute to advanced multi-modal tissue diagnostics.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0278-0062
• Sprache: Englisch