Identification of Pseudomonas asiatica subsp. bavariensis str. JM1 as the first N-epsilon-carboxy(m)ethyllysine-degrading soil bacterium

Mehler, Judith; Behringer, Kim Ina; Rollins, Robert Ethan; Pisarz, Friederike; Klingl, Andreas; Henle, Thomas; Heermann, Ralf; Becker, Noemie S.; Hellwig, Michael und Lassak, Jürgen (2022): Identification of Pseudomonas asiatica subsp. bavariensis str. JM1 as the first N-epsilon-carboxy(m)ethyllysine-degrading soil bacterium. In: Environmental Microbiology, Bd. 24, Nr. 7: S. 3229-3241

Abstract

Thermal food processing leads to the formation of advanced glycation end products (AGE) such as N-epsilon-carboxymethyllysine (CML). Accordingly, these non-canonical amino acids are an important part of the human diet. However, CML is only partially decomposed by our gut microbiota and up to 30% are excreted via faeces and, hence, enter the environment. In frame of this study, we isolated a soil bacterium that can grow on CML as well as its higher homologue N-epsilon-carboxyethyllysine (CEL) as sole source of carbon. Bioinformatic analyses upon whole-genome sequencing revealed a subspecies of Pseudomonas asiatica, which we named 'bavariensis'. We performed a metabolite screening of P. asiatica subsp. bavariensis str. JM1 grown either on CML or CEL and identified N-carboxymethylaminopentanoic acid and N-carboxyethylaminopentanoic acid respectively. We further detected alpha-aminoadipate as intermediate in the metabolism of CML. These reaction products suggest two routes of degradation: While CEL seems to be predominantly processed from the alpha-C-atom, decomposition of CML can also be initiated with cleavage of the carboxymethyl group and under the release of acetate. Thus, our study provides novel insights into the metabolism of two important AGEs and how these are processed by environmental bacteria.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Biologie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• ISSN: 1462-2912
• Sprache: Englisch