Capillimicrobium parvumgen. nov., sp. nov., a novel representative of Capillimicrobiaceae fam. nov. within the order Solirubrobacterales, isolated from a grassland soil

Vieira, Selma; Huber, Katharina J.; Geppert, Alicia; Wolf, Jacqueline; Neumann-Schaal, Meina; Luckner, Manja; Wanner, Gerhard; Muesken, Mathias und Overmann, Joerg (2022): Capillimicrobium parvumgen. nov., sp. nov., a novel representative of Capillimicrobiaceae fam. nov. within the order Solirubrobacterales, isolated from a grassland soil. In: International Journal of Systematic and Evolutionary Microbiology, Bd. 72, Nr. 8, 5508

Abstract

The order Solirubrobacterales is a deep-branching lineage within the phylum Actinomycetota. Most representatives have been isolated from terrestrial environments. A strain isolated from a grassland soil was found to be affiliated with this order and therefore characterized by a polyphasic approach. Cells of strain 0166_1(T) are Gram-positive, short rods, non-motile, non-spore-forming and divide by binary fission. A surface layer with protrusions covers the majority of the cells. Strain 0166_1(T) grows optimally around neutral to slightly alkaline pH (pH 7.1-7.9) and at temperatures between 24-36 degrees C in SSE/HD 1 : 10 medium. It grows optimally with 0-0.5% NaCl (w/v) but can withstand concentrations up to 5 %. The major fatty acids are C-18 : 1 omega 9c, C-16 : 1 omega 7c, C-17 : 0 cyclo omega 7c, C-18 : 1 omega 7c methyl and C-19 : 0 cyclo omega 9c. The major polar lipids are diphosphatidylglycerol, two unidentified phospholipids and one unidentified glycolipid. MK-7(H4) and MK-7(H2) are the predominant respiratory quinones. meso-2,6-Diaminopimelic acid is the diagnostic diamino acid in the cell-wall peptidoglycan. The G+C content for strain 0166_1(T) is 72.8 mol%. 16S rRNA gene sequence analysis indicated that this bacterium was related to Conexibacter arvalis KV-962(T) and Conexibacter stalactiti YC2-25(T) with 95.5 and 95.2 % sequence similarity, respectively. Based on the phenotypic, genomic and phylogenetic data, we propose the novel species Capillimicrobium parvum sp. nov. (type strain 0166_1(T)=DSM 104329(T)=LMG 29999(T)=CECT 9240(T)) of the novel genus Capillimicrobium gen. nov. within the novel family Capillimicrobiaceae fam. nov.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Biologie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• ISSN: 1466-5026
• Sprache: Englisch