Abstract
In regenerative medicine, noncontact manipulation of cells enables new possibilities for tissue engineering. Due to their physicochemical properties, superparamagnetic iron oxide nanoparticles (SPIONs) are used in biomedicine for various applications, e.g., as drug transporters, contrast agents or to make cells maneuverable by magnetic forces. SPIONs attached to and/or taken up by cells enable their magnetic targeting for adoptive immune therapies or tissue engineering. Remote control of different magnetized cell types can be used to construct multilayered tissues without the need for a scaffold structure. Here, the suitability of SPIONs with various coatings, such as polyacrylic acid-co-maleic acid (PAM), lauric acid (LA), lauric acid-human serum albumin (HSA), and citrate to magnetize cells is compared with the commercially available NanoShuttle-PL, designed for use in magnetic 3D cell cultures. Depending on the amount of cellular labeling, magnetic control is more or less effective. In particular, PAM- and citrate-coated SPIONs achieve good cellular loading and provide magnetic controllability of cells. In 2D cell culture, the magnetic cargo allows the patterned culture of cells. In 3D, SPIONs enable and accelerate spheroid formation as well as micropatterning using unloaded and loaded cells in parallel.
| Dokumententyp: | Zeitschriftenartikel |
|---|---|
| Fakultät: | Medizin |
| Themengebiete: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit |
| ISSN: | 1616-301X |
| Sprache: | Englisch |
| Dokumenten ID: | 115326 |
| Datum der Veröffentlichung auf Open Access LMU: | 02. Apr. 2024, 08:12 |
| Letzte Änderungen: | 02. Apr. 2024, 08:12 |
| DFG: | Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 450993414 |
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