ORCID: https://orcid.org/0000-0003-0378-4659; Salvermoser, Michael
ORCID: https://orcid.org/0000-0001-9412-8483; Theodorou, Johanna
ORCID: https://orcid.org/0000-0002-7841-1649; Böck, Andreas
ORCID: https://orcid.org/0000-0002-4511-7769; Sattler, Franziska; Kulig, Paulina; Tosevski, Vinko und Schaub, Bianca
ORCID: https://orcid.org/0000-0003-1652-8873
(14. Oktober 2024):
Farm‐dust mediated protection of childhood asthma: Mass cytometry reveals novel cellular regulation.
In: Allergy, Bd. 79, Nr. 11: S. 3022-3035
[PDF, 11MB]

Abstract
Background:
Farm-dust mediated asthma protection in childhood was replicated in numerous epidemiological studies. Central immune mechanisms are not fully understood. This exploratory study aimed to disentangle underlying immunological regulation of farm-dust mediated protection in peripheral blood on a single-cell level.
Methods:
Single-cell protein expression of in vitro farm-dust stimulated and unstimulated cells from allergic asthmatics and healthy controls were measured using mass cytometry. Analysis of innate and adaptive cellular proportions (linear regression) and T-cell proliferation was performed. Functional marker intensity was investigated using Earth Mover's Distance and the Monte Carlo permutation test.
Results:
Farm-dust stimulation induced cell type-specific regulation: Key-features of farm-dust stimulation comprised opposing regulation of immune-cell frequencies (downregulated innate cell populations (monocytes/DCs (p < .001), NK-cells (p < .05)) and upregulated adaptive populations (B-cells, CD4+ T-cells (both p < .05)), reduced CD4+ CD25− T-cell proliferation, and differential cell type-specific functional marker expression. Following stimulation, functional marker analysis revealed induced activation (CD25) in T-cells and NK-T-cells in both phenotypes even after correction for multiple testing. Cytotoxicity (GZMB) and inflammation (pERK1/2, pp38) related markers were reduced in T-cells exclusively in asthmatic children. Asthma-associated markers (Gata3, RORγ, and HLA-DR) were reduced in T- and innate- cell populations of asthmatics following stimulation. B-cells displayed a phenotypically independent increase of diverse functional markers upon farm-dust stimulation.
Conclusions:
This study mimicking in vivo environmental exposure identified a novel profile of immune-regulatory markers using mass cytometry demonstrating decreased asthma-associated markers following farm-dust stimulation. These findings may be key for further studies on asthma prevention in childhood.
Dokumententyp: | Zeitschriftenartikel |
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Fakultät: | Medizin > Institut für Diagnostische und Interventionelle Neuroradiologie
Medizin > Klinikum der LMU München > Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital |
Themengebiete: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit |
URN: | urn:nbn:de:bvb:19-epub-125649-7 |
ISSN: | 0105-4538 |
Sprache: | Englisch |
Dokumenten ID: | 125649 |
Datum der Veröffentlichung auf Open Access LMU: | 29. Apr. 2025 05:57 |
Letzte Änderungen: | 29. Apr. 2025 05:57 |
DFG: | Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 457276248 |
DFG: | Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 497431562 |