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Gardner, A.; Odebralski, J.; Zahler, Stefan; Korman, R. Z. and Aronson, A. I. (1982): Glutamine synthetase subunit mixing and regulation in Bacillus subtilis partial diploids. In: Journal of Bacteriology, Vol. 149, No. 1: pp. 378-380 [PDF, 476kB]

Abstract

A specialized transducing phage, SPβ c2 dglnA2, of Bacillus subtilis was used to construct partial diploids with various glutamine auxotrophs. The overproduction of manganese-stimulated glutamine synthetase no longer occurred in the diploids. The kinetics of heat inactivation of the enzyme extracted from two diploids suggests that there was subunit mixing.

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