Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1

Boeke, Joern; Regnard, Catherine; Cai, Weili; Johansen, Jørgen; Johansen, Kristen M; Becker, Peter B. und Imhof, Axel (6. April 2010): Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1.
In: PLOS ONE 5(4), e10042

Abstract

The histone methyltransferase SU(VAR)3-9 plays an important role in the formation of heterochromatin within the eukaryotic nucleus. Several studies have shown that the formation of condensed chromatin is highly regulated during development, suggesting that SU(VAR)3-9's activity is regulated as well. However, no mechanism by which this may be achieved has been reported so far. As we and others had shown previously that the N-terminus of SU(VAR)3-9 plays an important role for its activity, we purified interaction partners from Drosophila embryo nuclear extract using as bait a GST fusion protein containing the SU(VAR)3-9 N-terminus. Among several other proteins known to bind Su(VAR)3-9 we isolated the chromosomal kinase JIL-1 as a strong interactor. We show that SU(VAR)3-9 is a substrate for JIL-1 in vitro as well as in vivo and map the site of phosphorylation. These findings may provide a molecular explanation for the observed genetic interaction between SU(VAR)3-9 and JIL-1.

• Dokumententyp: Zeitschriftenartikel
• Publikationsform: Publisher's Version
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1932-6203
• Bemerkung: Work in the Imhof and Becker labs is supported by grants from the Deutsche Forschungsgemeinschaft [IM 23/4-3 and SFB-TR5 accordingly]. Work in the Johansen laboratory is supported by a grant from the National Institutes of Health [GM62916]
• Sprache: Englisch