Logo Logo
Help
Contact
Switch Language to German

Heil, Korbinian; Pearson, David and Carell, Thomas (2011): Chemical investigation of light induced DNA bipyrimidine damage and repair. In: Chemical Society Reviews, Vol. 40: pp. 4271-4278 [PDF, 1MB]

[thumbnail of Chemical_investigation_of_light_induced_DNA.pdf]
Preview
Download (1MB)

Abstract

In all organisms, genetic information is stored in DNA and RNA. Both of these macromolecules are damaged by many exogenous and endogenous events, with UV irradiation being one of the major sources of damage. The major photolesions formed are the cyclobutane pyrimidine dimers (CPD), pyrimidine–pyrimidone-(6-4)-photoproducts, Dewar valence isomers and, for dehydrated spore DNA, 5-(a-thyminyl)-5,6-dihydrothymine (SP). In order to be able to investigate how nature’s repair and tolerance mechanisms protect the integrity of genetic information, oligonucleotides containing sequence and site-specific UV lesions are essential. This tutorial review provides an overview of synthetic procedures by which these oligonucleotides can be generated, either through phosphoramidite chemistry or direct irradiation of DNA. Moreover, a brief summary on their usage in analysing repair and tolerance processes as well as their biological effects is provided.

Actions (login required)

View Item View Item