LC-MS based quantification of 2’-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

Pearson, David; Hienzsch, Antje; Wagner, Mirko; Globisch, Daniel; Reiter, Veronika; Özden, Dilek and Carell, Thomas (2011): LC-MS based quantification of 2’-ribosylated nucleosides Ar(p) and Gr(p) in tRNA. In: Chemical communications, Vol. 47: pp. 5196-5198

Abstract

RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2'-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi.

• Item Type: Journal article
• Faculties: Chemistry and Pharmacy
• Subjects: 500 Science > 540 Chemistry
• ISSN: 1359-7345
• Alliance/National Licence: This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
• Language: English