Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution?

Müther, Nadine; Noske, Nadja and Ehrhardt, Anja (December 2009): Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution? In: Viruses, Vol. 1, No. 3: pp. 1295-1324

Abstract

The turbulent history of clinical trials in viral gene therapy has taught us important lessons about vector design and safety issues. Much effort was spent on analyzing genotoxicity after somatic integration of therapeutic DNA into the host genome. Based on these findings major improvements in vector design including the development of viral hybrid vectors for somatic integration have been achieved. This review provides a state-of-the-art overview of available hybrid vectors utilizing viruses for high transduction efficiencies in concert with various integration machineries for random and targeted integration patterns. It discusses advantages but also limitations of each vector system.

• Item Type: Journal article
• Form of publication: Publisher's Version
• Keywords: hybrid vector; somatic integration; adenovirus; retrovirus; adeno-associated virus; herpes simplex virus; targeted integration; Sleeping Beauty transposase; PhiC31 integrase; zinc-finger nuclease
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 1999-4915
• Place of Publication: KANDERERSTRASSE 25, CH-4057 BASEL, SWITZERLAND
• Language: English