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Kalatskaya, I.; Schüssler, S.; Seidl, C.; Jochum, M.; Faussner, A. (2006): C-terminal fusion of eGFP to the bradykinin B-2 receptor strongly affects down-regulation but not receptor internalization or signaling. In: Biological Chemistry, Vol. 387, Nr. 5: S. 603-610
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Abstract

A functional comparison was made between the wildtype bradykinin B, receptor (B(2)wt) and the chimera B(2)eGFP (enhanced green-fluorescent protein fused to the C-terminus of B(2)Wt), both stably expressed in HEK 293 cells. There was almost no difference in terms of ligand-inducible receptor phosphorylation and internalization, signal transduction (accumulation of inositol phosphates) or expression and affinity. However, stimulation for up to 8 h with 10 mu M bradykinin (BK) resulted in a strong decrease in surface receptors (by 60% within 5 h) in B(2)Wt, but not in B(2)eGFP. When the expression levels of both constructs where comparably reduced using a weaker promoter, long-term stimulation resulted in a reduction in surface receptors for B(2)wt(low) to less than 20% within 1 h, whereas the chimera B(2)eGFP(low) still displayed 50% binding activity after 2 h. A 1-h incubation in the absence of BK resulted in a recovery of 60% of the binding in B(2)wt(low) after 1-h stimulation with BK, but of only 20% after 7-h stimulation. In contrast, B(2)eGFP(low) levels were restored to more than 70%, even after 7-h stimulation. These data indicate that although the fusion of eGFP to B(2)wt does not affect its ligand-induced internalization, it strongly reduces the down-regulation, most likely by promoting receptor recycling over degradation.