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Siess, Elmar A.; Hofstetter, Monika M. (2005): Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects. In: Biological Chemistry, Vol. 386, No. 11: pp. 1197-1201




A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S-400, chromatography on DEAE-cellulose and affinity chromatography on calmodulin-agarose. From the parallel change in staining intensity and in the level of the specific activity of enzyme fractions, a relationship between a 90-kDa SDS gel band, identified as the beta-isoform of the 90-kDa heat shock protein, and Mg2+-PAP could be detected.