Logo Logo
Hilfe
Hilfe
Switch Language to English

Vogeser, Michael und Spöhrer, Ute (2005): Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry. In: Clinical Chemistry and Laboratory Medicine, Bd. 43, Nr. 4: S. 400-402 [PDF, 101kB]

[thumbnail of cclm.2005.072.pdf]
Vorschau
Download (101kB)

Abstract

In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized in vivo; in liquid chromatography-tandem mass spectrometry respective metabolites can give rise to both parent and product ions that are isobaric with ions commonly used for the detection of cyclosporin A and cyclosporin D, respectively. In this article it is demonstrated that limited chromatography with co-elution of such metabolites together with cyclosporin A and cyclosporin D can lead to incorrect results.

Dokument bearbeiten Dokument bearbeiten