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Fraunberger, Peter; Pfeiffer, Matthias; Cremer, Peter; Holler, Ernst; Nagel, Dorothea; Dehart, Isabelle; Thein, Michael; Walli, Autar K.; Seidel, Dietrich (1998): Validation of an automated enzyme immunoassay for interleukin-6 for routine clinical use. In: Clinical Chemistry and Laboratory Medicine, Vol. 36, Nr. 10: S. 797-801
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Abstract

Serum levels of Interleukin-6 (IL-6), a proinflammatory cytokine, are increased in early stages of inflammatory diseases such as infection and sepsis. Assay systems which permit its measurement within a few hours and as a single measurement have not been reported so far. We therefore evaluated a now commercially available automated method for IL-6 measurement on the Cobas Core(R) immunological analyzer (Roche Diagnostic Systems) which enables single IL-6 measurement within about 1 hour. The automated assay correlates well with an established, manual microtiter plate assay (Biosource GmbH) which uses the same antibodies and reagents (r=0.98). Accuracy of the automated method was established by adding known amounts of IL-6 international reference preparation. Recovery of the international standard was in the range of 92-104%. The automated assay had a precision of singletons below 6% and was linear up to 2800 pg/ml. This automated assay provides a suitable, convenient and time saving method for measurement of IL-6 serum levels in the routine clinical laboratory.