Synthesis of gamma-labeled nucleoside 5 `-triphosphates using click chemistry

Serdjukow, Sascha; Kink, F.; Steigenberger, B.; Tomas-Gamasa, Maria und Carell, Thomas (20. December 2013): Synthesis of gamma-labeled nucleoside 5 `-triphosphates using click chemistry. In: Chemical communications, Vol. 50, No. 15: pp. 1861-1863

Abstract

Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of gamma-alkyne modified triphosphate amidates that are converted into a variety of gamma-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.

• Item Type: Journal article
• Form of publication: Publisher's Version
• Faculties: Chemistry and Pharmacy
• Subjects: 500 Science > 540 Chemistry
• ISSN: 0022-4936
• Alliance/National Licence: This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
• Annotation: First publ. online. Print publ. 2014
• Language: English