
Abstract
Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of gamma-alkyne modified triphosphate amidates that are converted into a variety of gamma-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.
Item Type: | Journal article |
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Form of publication: | Publisher's Version |
Faculties: | Chemistry and Pharmacy |
Subjects: | 500 Science > 540 Chemistry |
URN: | urn:nbn:de:bvb:19-epub-22638-2 |
ISSN: | 0022-4936 |
Alliance/National Licence: | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. |
Annotation: | First publ. online. Print publ. 2014 |
Language: | English |
Item ID: | 22638 |
Date Deposited: | 03. Feb 2015, 08:10 |
Last Modified: | 04. Nov 2020, 13:03 |