
Abstract
The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(alpha-thyminyl)-5,6-dihydrothymidine. Here we show that the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction.
Item Type: | Journal article |
---|---|
Form of publication: | Publisher's Version |
Faculties: | Chemistry and Pharmacy |
Subjects: | 500 Science > 540 Chemistry |
URN: | urn:nbn:de:bvb:19-epub-22693-2 |
ISSN: | 1359-7345 |
Alliance/National Licence: | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. |
Annotation: | First publ. online. Print publ. 2013 |
Language: | English |
Item ID: | 22693 |
Date Deposited: | 04. Feb 2015, 09:34 |
Last Modified: | 04. Nov 2020, 13:03 |