The radical SAM enzyme spore photoproduct lyase employs a tyrosyl radical for DNA repair

Kneuttinger, Andrea Christa; Heil, Korbinian; Kashiwazaki, Gengo und Carell, Thomas (28. November 2012): The radical SAM enzyme spore photoproduct lyase employs a tyrosyl radical for DNA repair. In: Chemical Communications, Vol. 49, No. 7: pp. 722-724

Abstract

The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(alpha-thyminyl)-5,6-dihydrothymidine. Here we show that the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction.

• Item Type: Journal article
• Form of publication: Publisher's Version
• Faculties: Chemistry and Pharmacy
• Subjects: 500 Science > 540 Chemistry
• ISSN: 1359-7345
• Alliance/National Licence: This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
• Annotation: First publ. online. Print publ. 2013
• Language: English