Gattner, Michael J.; Vrabel, Milan; Carell, Thomas
(15. November 2012):
Synthesis of epsilon-N-propionyl-, epsilon-N-butyryl-, and epsilon-N-crotonyl-lysine containing histone H3 using the pyrrolysine system.
In: Chemical Communications, Vol. 49, Nr. 4: S. 379-381
Recently new lysine modifications were detected in histones and other proteins. Using the pyrrolysine amber suppression system we genetically inserted three of the newamino acids epsilon-N-propionyl-, epsilon-N-butyryl-, and epsilon-N-crotonyl-lysine site specifically into histone H3. The lysine at position 9 (H3 K9), which is known to be highly modified in chromatin, was replaced by these unnatural amino acids.